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通过多位点酶电泳和十二烷基硫酸钠-聚丙烯酰胺凝胶电泳鉴别口腔白色念珠菌和都柏林念珠菌

Oral Candida albicans and Candida dubliniensis differentiation by multilocus enzyme electrophoresis and sodium dodecylsulphate-polyacrylamide gel electrophoresis.

作者信息

Rosa E A, Rosa R T, Boriollo M F, Bernardo W L, Höfling J F

机构信息

Stomatology Laboratory, Center of Biological and Health Sciences, Pontifical Catholic University of Paraná, Avenida Imaculada Conceição, 1155-Prado Velho-CEP 80215-901, Curitiba, Brazil.

出版信息

Rev Argent Microbiol. 2003 Jan-Mar;35(1):24-8.

PMID:12833677
Abstract

Five oral strains of Candida albicans and five C. dubliniensis, as well as their respective type-strains, were analyzed by multilocus enzyme electrophoresis (MLEE) and sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE). After electrophoreses and numerical analyses, we obtained two distinct species-specific taxa, which may justify the use of MLEE and SDS-PAGE as reliable methods for differentiation and complementary identification of C. dubliniensis.

摘要

通过多位点酶电泳(MLEE)和十二烷基硫酸钠聚丙烯酰胺凝胶电泳(SDS-PAGE)对五株白色念珠菌口腔菌株、五株都柏林念珠菌及其各自的模式菌株进行了分析。经过电泳和数值分析,我们获得了两个不同的物种特异性分类群,这可能证明MLEE和SDS-PAGE作为区分和补充鉴定都柏林念珠菌的可靠方法是合理的。

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