Rosa E A, Rosa R T, Boriollo M F, Bernardo W L, Höfling J F
Stomatology Laboratory, Center of Biological and Health Sciences, Pontifical Catholic University of Paraná, Avenida Imaculada Conceição, 1155-Prado Velho-CEP 80215-901, Curitiba, Brazil.
Rev Argent Microbiol. 2003 Jan-Mar;35(1):24-8.
Five oral strains of Candida albicans and five C. dubliniensis, as well as their respective type-strains, were analyzed by multilocus enzyme electrophoresis (MLEE) and sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE). After electrophoreses and numerical analyses, we obtained two distinct species-specific taxa, which may justify the use of MLEE and SDS-PAGE as reliable methods for differentiation and complementary identification of C. dubliniensis.
通过多位点酶电泳(MLEE)和十二烷基硫酸钠聚丙烯酰胺凝胶电泳(SDS-PAGE)对五株白色念珠菌口腔菌株、五株都柏林念珠菌及其各自的模式菌株进行了分析。经过电泳和数值分析,我们获得了两个不同的物种特异性分类群,这可能证明MLEE和SDS-PAGE作为区分和补充鉴定都柏林念珠菌的可靠方法是合理的。