Springfield E P, Amabeoku G, Weitz F, Mabusela W, Johnson Q
South African Traditional Medicines Research Group, School of Pharmacy, University of the Western Cape, Bellville, South Africa.
Phytomedicine. 2003;10(5):434-9. doi: 10.1078/0944-7113-00263.
For centuries, indigenous people in South Africa have used a variety of medicinal herbs to treat chronic infections. This investigation focused on two Carpobrotus species belonging to the family, Aizoaceae, in an attempt to assess their antimicrobial potential. Extracts of varying polarities of the plants were prepared and tested against Staphylococcus aureus, Pseudomonas aeruginosa, Candida albicans and Mycobacterium smegmatis. For the disc diffusion method, Ciprofloxacin (40 microg/disc) served as positive control for S. aureus, P. aeruginosa and M. smegmatis, whereas amphotericin B (25 microg/disc) was the control for C. albicans. A sample concentration of 10 mg/ml was used. Minimum inhibitory concentrations (MIC) were determined by two-fold serial dilution. Phytochemical analysis was completed to test for the presence of flavonoids, hydrolysable tannins, phytosterols and aromatic acids. The ethyl acetate extracts (21 microl of 95 mg/ml) were used for bio-autography, together with TLC analyses. Carpobrotus muirii and Carpobrotus quadrifidus showed antimicrobial activity against S. aureus and M. smegmatis in the disc diffusion method and inhibition against S. aureus and M. smegmatis was observed by clear zones on the TLC plate. This investigation confirms that extracts of these Carpobrotus species that are used as indigenous medicines, exhibit anti-bacterial activity. This scientific information can serve as an important platform for the development of inexpensive, safe and effective natural anti-infective medicines.
几个世纪以来，南非的原住民一直使用各种药草来治疗慢性感染。本研究聚焦于番杏科的两种日中花属植物，旨在评估它们的抗菌潜力。制备了不同极性的植物提取物，并针对金黄色葡萄球菌、铜绿假单胞菌、白色念珠菌和耻垢分枝杆菌进行测试。对于纸片扩散法，环丙沙星（40微克/纸片）用作金黄色葡萄球菌、铜绿假单胞菌和耻垢分枝杆菌的阳性对照，而两性霉素B（25微克/纸片）则作为白色念珠菌的对照。使用的样品浓度为10毫克/毫升。通过两倍系列稀释法测定最低抑菌浓度（MIC）。完成了植物化学分析以检测黄酮类化合物、可水解单宁、植物甾醇和芳香酸的存在。乙酸乙酯提取物（21微升，95毫克/毫升）与薄层色谱分析一起用于生物自显影。在纸片扩散法中，缪氏日中花和四裂日中花对金黄色葡萄球菌和耻垢分枝杆菌表现出抗菌活性，并且在薄层色谱板上通过透明圈观察到对金黄色葡萄球菌和耻垢分枝杆菌的抑制作用。本研究证实，这些用作本土药物的日中花属植物提取物具有抗菌活性。这一科学信息可为开发廉价、安全且有效的天然抗感染药物提供重要平台。
