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Synthesis of 3-O-beta-N-acetylglucosaminyl cyclic tetrasaccharide through a lysozyme-catalyzed transfer reaction.

作者信息

Watanabe Hikaru, Aga Hajime, Sonoda Tomohiko, Kubota Michio, Fukuda Shigeharu, Kurimoto Masashi, Tsujisaka Yoshio

机构信息

Amase Institute, Hayashibara Biochemical Laboratories, Inc., 7-7 Amase-minami machi, Okayama 700-0834, Japan.

出版信息

Biosci Biotechnol Biochem. 2003 May;67(5):1182-4. doi: 10.1271/bbb.67.1182.

DOI:10.1271/bbb.67.1182
PMID:12834307
Abstract

Egg white lysozyme was found to catalyze the transfer of N-acetylglucosamine to cyclo[-->6)-alpha-D-Glcp-(1-->3)-alpha-D-Glcp-(1-->6)-alpha-D-Glcp-(1-->3)-alpha-D-Glcp-(1-->] (CTS). Structural analysis showed that the transfer product was 3-O-beta-N-acetylglucosaminyl CTS, cyclo[-->6)-alpha-D-Glcp-(1-->3)-alpha-D-Glcp-(1-->6)-[beta-GlcNAc-(1-->3)]-alpha-D-Glcp-(1-->3)-alpha-D-Glcp-(1-->]. This branched saccharide is anticipated to be a model compound of the sugar chains of glycoproteins.

摘要

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