一种通过α-(1→6)键从直链淀粉酶促产生的新型环麦芽五糖，环-{→6)-α-D-葡萄糖吡喃糖基-(1→4)-α-D-葡萄糖吡喃糖基-(1→4)-α-D-葡萄糖吡喃糖基-(1→4)-α-D-葡萄糖吡喃糖基-(1→} 。

An enzymatically produced novel cyclomaltopentaose cyclized from amylose by an alpha-(1-->6)-linkage, cyclo-{-->6)-alpha-D-Glcp-(1-->4)-alpha-D-Glcp-(1-->4)-alpha-D-Glcp-(1-->4)-alpha-D-Glcp-(1-->4)-alpha-D-Glcp-(1-->}.

作者信息

Watanabe Hikaru, Nishimoto Tomoyuki, Sonoda Tomohiko, Kubota Michio, Chaen Hiroto, Fukuda Shigeharu

机构信息

Amase Institute, Hayashibara Biochemical Laboratories, Inc., 7-7 Amase minami-machi, Okayama 700-0834, Japan.

出版信息

Carbohydr Res. 2006 Jun 12;341(8):957-63. doi: 10.1016/j.carres.2006.02.028. Epub 2006 Mar 20.

DOI:10.1016/j.carres.2006.02.028

PMID:16545346

Abstract

A bacterial strain AM7, isolated from soil and identified as Bacillus circulans, produced two kinds of novel cyclic oligosaccharides. The cyclic oligosaccharides were produced from amylose using a culture supernatant of the strain as the enzyme preparation. The major product was a cyclomaltopentaose cyclized by an alpha-(1-->6)-linkage, cyclo-{-->6)-alpha-D-Glcp-(1-->4)-alpha-D-Glcp-(1-->4)-alpha-D-Glcp-(1-->4)-alpha-D-Glcp-(1-->4)-alpha-D-Glcp-(1-->}. The other minor product was cyclomaltohexaose cyclized by an alpha-(1-->6)-linkage, cyclo-{-->6)-alpha-D-Glcp-(1-->4)-alpha-D-Glcp-(1-->4)-alpha-D-Glcp-(1-->4)-alpha-D-Glcp-(1-->4)-alpha-D-Glcp-(1-->4)-alpha-D-Glcp-(1-->}. We propose the names isocyclomaltopentaose (ICG5) and isocyclomaltohexaose (ICG6) for these novel cyclic maltooligosaccharides having one alpha-(1-->6)-linkage. ICG5 was digested by alpha-amylase derived from Aspergillus oryzae, cyclomaltodextrin glucanotransferase (CGTase) from Bacillus stearothermophilus, and maltogenic alpha-amylase. On the other hand, ICG6 was digested by CGTase from B. stearothermophilus and B. circulans, and maltogenic alpha-amylase. This is the first report of enzymatically produced cyclomaltopentaose and cyclomaltohexaose, which have an alpha-(1-->6)-linkage in their molecules.

摘要

从土壤中分离出的一株编号为AM7的细菌，经鉴定为环状芽孢杆菌，它能产生两种新型环状寡糖。利用该菌株的培养上清液作为酶制剂，从直链淀粉中制备出了这些环状寡糖。主要产物是一种通过α-(1→6)键环化的环麦芽五糖，即环-{→6)-α-D-葡萄糖-(1→4)-α-D-葡萄糖-(1→4)-α-D-葡萄糖-(1→4)-α-D-葡萄糖-(1→4)-α-D-葡萄糖-(1→}。另一种次要产物是通过α-(1→6)键环化的环麦芽六糖，即环-{→6)-α-D-葡萄糖-(1→4)-α-D-葡萄糖-(1→4)-α-D-葡萄糖-(1→4)-α-D-葡萄糖-(1→4)-α-D-葡萄糖-(1→4)-α-D-葡萄糖-(1→}。对于这些具有一个α-(1→6)键的新型环状麦芽寡糖，我们提议将其命名为异环麦芽五糖（ICG5）和异环麦芽六糖（ICG6）。ICG5能被米曲霉来源的α-淀粉酶、嗜热脂肪芽孢杆菌的环糊精葡萄糖基转移酶（CGTase）以及产麦芽糖α-淀粉酶消化。另一方面，ICG6能被嗜热脂肪芽孢杆菌和环状芽孢杆菌的CGTase以及产麦芽糖α-淀粉酶消化。这是关于酶法制备分子中含有α-(1→6)键的环麦芽五糖和环麦芽六糖的首次报道。