Brenner R E, Riemenschneider B, Blum W, Mörike M, Teller W M, Pirsig W, Heinze E
Department of Pediatrics I, University of Ulm, Germany.
Acta Endocrinol (Copenh). 1992 Dec;127(6):509-14. doi: 10.1530/acta.0.1270509.
We studied the influence of fasting serum from nine insulin-dependent diabetic children and adolescents under insufficient metabolic control on normal human bone cells in vitro compared with serum from eight sex- and age-matched controls. Cell number 24 h after plating was significantly less under diabetic serum, indicating impaired cell attachment, spreading and initiation of cell proliferation. Cell number after five days was reduced by 1% diabetic serum, while higher serum concentrations had diverging effects on osteoblast proliferation. Collagen synthesis of human osteoblasts was significantly reduced by 8% diabetic serum compared to 8% control serum, while synthesis of non-collagenous proteins was not affected. Duration of diabetes (several weeks up to 12 years) had no influence on these parameters. The serum from one patient, which was studied a second time under excellent metabolic control three months later, however, had lost its inhibitory influence on collagen synthesis of osteoblasts. The pattern of the interstitial collagen types I, III and V was not altered by diabetic serum. These results indicate that defective regulation of proliferation and collagen synthesis of osteoblasts by components present in human diabetic serum may be an important factor in the development of diabetic osteopenia. The negative influence might be explained in part by reduced levels of IGF-I and elevated levels of IGF binding protein-1 in the diabetic sera.
我们研究了9名代谢控制不佳的胰岛素依赖型糖尿病儿童和青少年的空腹血清对体外正常人骨细胞的影响，并与8名性别和年龄匹配的对照组血清进行了比较。接种24小时后，糖尿病血清组的细胞数量显著减少，表明细胞附着、铺展及细胞增殖起始受损。5天后，1%糖尿病血清使细胞数量减少，而更高血清浓度对成骨细胞增殖有不同影响。与8%对照血清相比，8%糖尿病血清使人类成骨细胞的胶原蛋白合成显著减少8%，而非胶原蛋白合成未受影响。糖尿病病程（数周至12年）对这些参数无影响。然而，三个月后在良好代谢控制状态下对一名患者的血清进行再次研究时，其血清对成骨细胞胶原蛋白合成的抑制作用已消失。糖尿病血清未改变间质I型、III型和V型胶原蛋白的模式。这些结果表明，人糖尿病血清中存在的成分对成骨细胞增殖和胶原蛋白合成的调节缺陷可能是糖尿病性骨质减少发生的一个重要因素。这种负面影响可能部分由糖尿病血清中IGF-I水平降低和IGF结合蛋白-1水平升高来解释。
