Valér M
Berufsdermatosen. 1975 Jun;23(3):96-115.
The late type contact sensitizing effect of alkaline protease enzymes (PE) on the intact human skin has been investigated in the present study. The immune process of sensitisation was induced with "Tenzym prilled" (TP, Grindstedvoerket) and with "Maxatase" (M, Gist-Brocades) protease enzymes in the epicutaneous test (ET), using concentration series and various durations of application. The ETs were made on the intact (symptom-free) skin, as well as under conditions promoting the subcorneal penetration of PE. Challenge was carried out at 21 to 30 days following induction of 2092 subjects, and at 2 to 5 months on 1624 subjects. Despite the large number of subjects tested, contact sensitisation developed in none of the cases, although the inducing exposure took place under conditions promoting the immune process of sensitisation. In 60 individuals suffereing from occupational dermatitis on regular contact with PE and having no symptoms of early type inhalative allergy (mucous membrane changes, bronchial asthma-like symptoms) were challenged also by the intradermal test. No reaction was noted in any of them at 10 and 30 minutes, as well as at 24 and 48 hours following the test. Next the influence of PE is analysed in the induction or increased severity of the irritation caused by bioactive laundry detergents. The studies involved the use of serial dilutions of "Biopon" (Bn) laundry detergent containing TP or M, or not containing PE, respectively, by means of the ET. A total of 740 series (5220 tests) of the three variants were applied in dilution series to intact skin surface, as well as to pathologically and arteficially lsioned skin areas. The Bn variants containing and not containing PE increased the number of irratative reactions in essentially the same degree. This suggests that the irritative effect is not due to the presence of PE, but to the laundry-active detergents (WAS) of Bn in the first place, and to a lesser extent to its other ingredients.
本研究调查了碱性蛋白酶（PE）对完整人体皮肤的迟发型接触致敏作用。通过皮上试验（ET）用“Tenzym prilled”（TP，Grindstedvoerket）和“Maxatase”（M，Gist-Brocades）蛋白酶通过系列浓度和不同涂抹时间来诱导致敏的免疫过程。ET在完整（无症状）皮肤上进行，也在促进PE角质层下渗透的条件下进行。在2092名受试者诱导后21至30天进行激发试验，1624名受试者在2至5个月后进行激发试验。尽管测试了大量受试者，但在任何情况下均未发生接触致敏，尽管诱导暴露是在促进致敏免疫过程的条件下进行的。对60名经常接触PE且无早期吸入性过敏症状（粘膜变化、支气管哮喘样症状）的职业性皮炎患者也进行了皮内试验激发。在试验后10分钟、30分钟以及24小时和48小时，他们中没有人出现反应。接下来分析了PE对生物活性洗衣粉引起的刺激的诱导或加重程度的影响。这些研究通过ET使用了分别含有TP或M或不含PE的“Biopon”（Bn）洗衣粉的系列稀释液。总共740个系列（5220次试验）的三种变体以稀释系列应用于完整皮肤表面以及病理和人工损伤的皮肤区域。含PE和不含PE的Bn变体在基本上相同的程度上增加了刺激性反应的数量。这表明刺激作用不是由于PE的存在，首先是由于Bn的洗衣活性洗涤剂（WAS），其次在较小程度上是由于其其他成分。
