Strlic Matija, Kolar Jana
Faculty of Chemistry and Chemical Technology, University of Ljubljana, Askerceva 5, Ljubljana SI-1000, Slovenia.
J Biochem Biophys Methods. 2003 Jun 30;56(1-3):265-79. doi: 10.1016/s0165-022x(03)00064-2.
Size exclusion of cellulose in LiCl/N,N-dimethylacetamide has been used for the past 15 years, yet much of the current research is still devoted to fundamental studies, as many issues regarding column calibration, separation mechanisms and solution behavior have not been resolved yet. The paper reviews practical aspects of sample preparation and it is demonstrated that sample heating and several techniques to aid solvent exchange call for reevaluation. It is further shown that the use of internal standard may introduce minor improvements in repeatability. The commonly used column calibration procedures, chromatographic conditions and applications are also reviewed. Further research is needed to understand the mechanisms of separation, to optimize column calibration and to facilitate and optimize sample preparation.
在过去15年中，已采用尺寸排阻法在LiCl/N，N-二甲基乙酰胺中分离纤维素，然而目前许多研究仍致力于基础研究，因为有关柱校准、分离机制和溶液行为的许多问题尚未得到解决。本文回顾了样品制备的实际方面，并证明样品加热和几种辅助溶剂交换的技术需要重新评估。还进一步表明，使用内标可能会在重复性方面带来微小的改进。本文还回顾了常用的柱校准程序、色谱条件及应用。需要进一步开展研究，以了解分离机制、优化柱校准，并促进和优化样品制备。
