Eremeeva Tatyana
Latvian State Institute of Wood Chemistry, 27 Dzerbenes Str., LV 1006, Riga, Latvia.
J Biochem Biophys Methods. 2003 Jun 30;56(1-3):253-64. doi: 10.1016/s0165-022x(03)00063-0.
Size-exclusion chromatography (SEC) of wood pulp polysaccharides (cellulose and hemicelluloses) has become widely used for the analysis of these polymers, including monitoring of transformations occurring under various chemical and biological processes. The present review provides information on the different procedures and methodologies developed for the characterization of molecular mass distribution (MMD) and chemical composition of cellulose and hemicelluloses by SEC. This paper also includes the significant results obtained in the characterization of enzymatically treated wood polysaccharides. Conventional SEC with double detection (refractometric and UV), "hyphenated" with a fractional dissolution, was demonstrated to be an efficient method for simultaneous determination of MMD and chemical heterogeneity.
木浆多糖（纤维素和半纤维素）的尺寸排阻色谱法（SEC）已广泛用于这些聚合物的分析，包括监测在各种化学和生物过程中发生的转化。本综述提供了有关通过SEC表征纤维素和半纤维素的分子量分布（MMD）和化学组成所开发的不同程序和方法的信息。本文还包括酶处理木多糖表征中获得的重要结果。具有双检测（折光和紫外）的传统SEC与分步溶解“联用”，被证明是同时测定MMD和化学异质性的有效方法。
