Jobbágy A J, Aversa N O, Denoya C D
Biochem Genet. 1975 Dec;13(11-12):813-31. doi: 10.1007/BF00484413.
In an attempt to find clues for the significance of the gene ordering along the eukaryotic chromosome, a system consisting of germinating conida of Neurospora crassa was studied. Thirteen enzyme activities corresponding to genes widely distributed on five chromosomes were determined in dormant and in germinating conidia. Ten of these enzymes showed lower activities in the resting state, and the time patterns of their increase were determined during germination. The results obtained do not support a scheme of sequential expression of genes during the emergence from dormancy as a counterpart of the sequence of the corresponding genes along the chromosome. Two of the loci studied were analyzed both in the normal (wild-type) ordering and in a translocated position in which the two genes are located in an inverted order respective to the centromere and farther apart from it. The altered order of the genes did not influence significantly the time and pattern of increase in the activities of the corresponding enzymes.
为了寻找真核染色体上基因排列顺序具有重要意义的线索，对一个由粗糙脉孢菌的萌发分生孢子组成的系统进行了研究。在休眠和萌发的分生孢子中测定了与广泛分布在五条染色体上的基因相对应的13种酶活性。其中10种酶在静止状态下活性较低，并在萌发过程中确定了它们活性增加的时间模式。所获得的结果并不支持一种在从休眠中苏醒期间基因按顺序表达的模式，这种模式是与相应基因在染色体上的顺序相对应的。对所研究的两个基因座进行了分析，一个是正常（野生型）排列，另一个是易位位置，在易位位置上这两个基因相对于着丝粒呈反向排列且离着丝粒更远。基因顺序的改变并没有显著影响相应酶活性增加的时间和模式。
