通过光敏反应产生的单重态分子氧使粗糙脉孢菌分生孢子失活。
Inactivation of Neurospora crassa conidia by singlet molecular oxygen generated by a photosensitized reaction.
作者信息
Shimizu M, Egashira T, Takahama U
出版信息
J Bacteriol. 1979 May;138(2):293-6. doi: 10.1128/jb.138.2.293-296.1979.
Abstract
Photodynamic damage of Neurospora crassa conidia was studied in the presence of the photosensitizing dye, toluidine blue O. Conidia which germinated to form colonies decreased in number as irradiation time became longer. The photoinactivation of conidia was suppressed by azide, bovine serum albumin, and histidine, and was stimulated in deuterium oxide. Wild-type conidia were less sensitive to the irradiation than albino conidia. In the wild-type, carotenoid-enriched conidia were more resistant against the lethal damage than the conidia which contained small amounts of carotenoids. These results suggest that singlet molecular oxygen causes photodynamic lethal damage to N. crassa conidia and that singlet molecular oxygen is quenched by endogenous carotenoids.
摘要
在光敏染料甲苯胺蓝O存在的情况下，研究了粗糙脉孢菌分生孢子的光动力损伤。随着照射时间延长，萌发形成菌落的分生孢子数量减少。叠氮化物、牛血清白蛋白和组氨酸可抑制分生孢子的光灭活，而在重水中则受到刺激。野生型分生孢子比白化分生孢子对照射更不敏感。在野生型中，富含类胡萝卜素的分生孢子比含有少量类胡萝卜素的分生孢子对致死损伤更具抗性。这些结果表明，单线态分子氧对粗糙脉孢菌分生孢子造成光动力致死损伤，并且单线态分子氧被内源性类胡萝卜素淬灭。
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