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一种从亚线粒体颗粒制备三磷酸腺苷酶的简单快速方法。

A simple and rapid method for the preparation of adenosine triphosphatase from submitochondrial particles.

作者信息

Beechey R B, Hubbard S A, Linnett P E, Mitchell A D, Munn E A

出版信息

Biochem J. 1975 Jun;148(3):533-7. doi: 10.1042/bj1480533.

DOI:10.1042/bj1480533
PMID:128353
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1165573/
Abstract

An almost pure form of the bovine heart mitochondrial adenosine triphosphatase (ATPase) is released from the membrane by shaking submitochondrial particles with chloroform. Analyses on polyacrylamide gels and by electron microscopy, and also sensitivity to inhibitors, show that the chloroform-released enzyme is similar to other ATPase preparations from bovine heart mitochondria.

摘要

通过用氯仿振荡亚线粒体颗粒，可从膜中释放出几乎纯形式的牛心线粒体腺苷三磷酸酶（ATP酶）。聚丙烯酰胺凝胶分析、电子显微镜分析以及对抑制剂的敏感性表明，氯仿释放的酶与来自牛心线粒体的其他ATP酶制剂相似。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/253d/1165573/47df740fcf65/biochemj00556-0195-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/253d/1165573/4277dbec133c/biochemj00556-0194-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/253d/1165573/47df740fcf65/biochemj00556-0195-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/253d/1165573/4277dbec133c/biochemj00556-0194-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/253d/1165573/47df740fcf65/biochemj00556-0195-a.jpg

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