牛心亚线粒体颗粒中三磷酸腺苷酶抑制剂的含量。抑制剂对三磷酸腺苷依赖性反应的影响。
The adenosine triphosphatase-inhibitor content of bovine heart submitochondrial particles. Influence of the inhibitor on adenosine triphosphate-dependent reactions.
作者信息
Ferguson S J, Harris D A, Radda G K
出版信息
Biochem J. 1977 Feb 15;162(2):351-7. doi: 10.1042/bj1620351.
Abstract
- The activity of the ATPase (adenosine triphosphatase) of phosphorylating particles prepared by sonication of bovine heart mitochondria in the presence of MgCl2 and ATP is influenced by the isolation method for the mitochondria used in the preparation of particles. Type-I particles, made from mitochondria isolated in a medium lacking succinate, have a lower ATPase activity than to Type-II particles, which are prepared from mitochondria isolated in a medium containing succinate. 2. Centrifugation under appropriate energized conditions increases the ATPase activity of Type-I particles almost to that of the Type-II particles. The ATPase activity of Type-II particles was only slightly stimulated by this procedure. These data are interpreted as indicating a higher content of the ATPase-inhibitor protein in the Type-I particles. 3. A comparison was made of the ATP-driven enhancement of 8-anilinonaphthalene-1-sulphonate fluorescence and the exchange of the endogenous tightly bound nucleotides of the ATPase in Type-I and Type-II particles. The effect of exogenous inhibitor protein on both these reactions was also studied. 4. The time-scale on which the inhibitor protein can exchange between ATPase molecules is discussed.
摘要
- 在MgCl₂和ATP存在的情况下，通过超声处理牛心线粒体制备的磷酸化颗粒的ATP酶（三磷酸腺苷酶）活性受颗粒制备过程中线粒体分离方法的影响。由在缺乏琥珀酸的培养基中分离的线粒体制成的I型颗粒，其ATP酶活性低于由在含有琥珀酸的培养基中分离的线粒体制备的II型颗粒。2. 在适当的通电条件下离心可使I型颗粒的ATP酶活性几乎提高到II型颗粒的水平。此程序对II型颗粒的ATP酶活性仅略有刺激。这些数据被解释为表明I型颗粒中ATP酶抑制蛋白的含量更高。3. 对I型和II型颗粒中ATP驱动的8-苯胺基萘-1-磺酸盐荧光增强以及ATP酶内源性紧密结合核苷酸的交换进行了比较。还研究了外源性抑制蛋白对这两个反应的影响。4. 讨论了抑制蛋白在ATP酶分子之间交换的时间尺度。
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