Kumar Justin P, Hsiung Frank, Powers Maureen A, Moses Kevin
Department of Cell Biology, Emory University School of Medicine, Atlanta, GA 30322, USA.
Development. 2003 Aug;130(16):3703-14. doi: 10.1242/dev.00556.
In proneural groups of cells in the morphogenetic furrow of the developing Drosophila eye phosphorylated mitogen activated protein kinase (MAPK) antigen is held in the cytoplasm for hours. We have developed a reagent to detect nuclear MAPK non-antigenically and report our use of this reagent to confirm that MAPK nuclear translocation is regulated by a second mechanism in addition to phosphorylation. This "cytoplasmic hold" of activated MAPK has not been observed in cell culture systems. We also show that MAPK cytoplasmic hold has an essential function in vivo: if it is overcome, developmental patterning in the furrow is disrupted.
在发育中的果蝇眼睛形态发生沟的神经前体细胞群中，磷酸化的丝裂原活化蛋白激酶（MAPK）抗原在细胞质中停留数小时。我们开发了一种试剂，用于非抗原性地检测细胞核中的MAPK，并报告我们使用该试剂来证实，除了磷酸化之外，MAPK的核转位还受另一种机制调控。在细胞培养系统中尚未观察到活化的MAPK这种“细胞质滞留”现象。我们还表明，MAPK的细胞质滞留在体内具有重要功能：如果这种滞留被克服，沟中的发育模式就会被破坏。
