Xie Linglin, Gao Qiuhua, Xu Huibi
Family Planning Research Institute of Tongji Medical College, Wuhan 430074, China.
Biol Trace Elem Res. 2003 Summer;93(1-3):227-36. doi: 10.1385/BTER:93:1-3:227.
The present investigation was carried out to study the ameliorative effects of L-Met (L-methionine) on Pb-exposed mice by the measurement of the weight gain, the weight of organs, the Hb content, the level of lipid peroxidation (LPO), and the Pb and Fe contents of selected organs and serum. Thirty mice were randomly divided into the healthy control group (A), the Pb control group (B), and the Pb+L-Met-treated group (C). Lead-induced depression of growth and Hb content was reversed when L-Met was added to the daily drinking water. The level of LPO of all the samples from group B was significantly higher than group A (p<0.05); however, in group C, it was lowered, especially that in the brain, kidney, and serum, which were not significantly different than normal (p>0.05). Significant enhancement of Pb content (p<0.05) and significant decrease of Fe content of all the samples in group B (p<0.05) were observed. However, dietary Met significantly lowered Pb concentration of all the samples (p<0.05) compared to that of the liver and kidney, reaching no significance in comparison to group A (p>0.05). The Fe concentration changed little (p>0.05). The result suggests that L-Met has an ameliorative effect on Pb-exposed mice.
本研究旨在通过测量体重增加、器官重量、血红蛋白含量、脂质过氧化水平（LPO）以及所选器官和血清中的铅和铁含量，研究L-蛋氨酸（L-甲硫氨酸）对铅暴露小鼠的改善作用。30只小鼠被随机分为健康对照组（A组）、铅对照组（B组）和铅+L-蛋氨酸治疗组（C组）。当将L-蛋氨酸添加到日常饮用水中时，铅诱导的生长抑制和血红蛋白含量降低得到逆转。B组所有样本的LPO水平均显著高于A组（p<0.05）；然而，C组的LPO水平降低，尤其是脑、肾和血清中的LPO水平，与正常水平无显著差异（p>0.05）。观察到B组所有样本的铅含量显著升高（p<0.05），铁含量显著降低（p<0.05）。然而，与肝脏和肾脏相比，饮食中的蛋氨酸显著降低了所有样本中的铅浓度（p<0.05），与A组相比无显著性差异（p>0.05）。铁浓度变化不大（p>0.05）。结果表明，L-蛋氨酸对铅暴露小鼠具有改善作用。
