Kanazawa Yusei, Isomoto Hajime, Oka Mikio, Yano Yoshitsugu, Soda Hiroshi, Shikuwa Saburo, Takeshima Fuminao, Omagari Katsuhisa, Mizuta Yohei, Murase Kunihiko, Nakagoe Toru, Ohtsuka Kenzo, Kohno Shigeru
Second Department of Internal Medicine, Nagasaki University School of Medicine, 1-7-1 Sakamoto, Nagasaki 852-8501, Japan.
Med Oncol. 2003;20(2):157-64. doi: 10.1385/MO:20:2:157.
Heat shock protein (Hsp) 70 and Hsp 40 are stress proteins that cooperate as chaperones in mammalian cells. The present study was designed to determine the expression levels of Hsp 70 and Hsp 40 in colorectal cancer by immunohistochemistry and Western blot analysis. Among 50 colorectal cancer tissues studied, 80% and 14% of tumors showed specific immunoreactivity to Hsp 70 and Hsp 40, respectively. Hsp 70 and Hsp 40 were overexpressed in cancer tissue samples compared with normal tissues, on both analytic sets. However, there were no significant correlations between their expression and various clinicopathological parameters of colorectal cancer. Hsp 70 and Hsp 40 may be tumor markers for colorectal cancer.
热休克蛋白（Hsp）70和Hsp 40是在哺乳动物细胞中作为伴侣蛋白协同作用的应激蛋白。本研究旨在通过免疫组织化学和蛋白质印迹分析来确定Hsp 70和Hsp 40在结直肠癌中的表达水平。在所研究的50例结直肠癌组织中，分别有80%和14%的肿瘤对Hsp 70和Hsp 40表现出特异性免疫反应性。在两个分析组中，与正常组织相比，癌组织样本中Hsp 70和Hsp 40均过表达。然而，它们的表达与结直肠癌的各种临床病理参数之间无显著相关性。Hsp 70和Hsp 40可能是结直肠癌的肿瘤标志物。
