Oka M, Sato S, Soda H, Fukuda M, Kawabata S, Nakatomi K, Shiozawa K, Nakamura Y, Ohtsuka K, Kohno S
Division of Molecular and Clinical Microbiology, Department of Molecular Microbiology and Immunology, Nagasaki University Graduate School of Medical Sciences, Nagasaki 852-8523, Japan.
Jpn J Cancer Res. 2001 Mar;92(3):316-20. doi: 10.1111/j.1349-7006.2001.tb01097.x.
Heat shock protein Hsp40 is a stress protein with chaperone activity and has a cooperative function with Hsp70 in mammalian cells. We examined the possible expression of Hsp40 in lung tumor tissues using immunoblotting and immunohistochemistry, and established an enzyme-linked immunosorbent assay (ELISA) method to detect IgG antibody to Hsp40 in the serum using purified human Hsp40. Sera were obtained from 130 normal subjects and 50 patients with lung cancer. Lung tumor tissues and cells specifically overexpressed Hsp40, and no such expression was detected in normal lung tissues. Compared with normal sera, significantly higher levels of autoantibody to Hsp40 were present in patients with lung cancer. The present study is the first to demonstrate overexpression of Hsp40 in human tumor tissue and the associated presence of autoantibody to Hsp40 in the serum. These results suggest that overexpression of Hsp40 in tumor cells may be recognized as a self-antigen.
热休克蛋白Hsp40是一种具有伴侣活性的应激蛋白，在哺乳动物细胞中与Hsp70具有协同功能。我们使用免疫印迹和免疫组织化学方法检测了Hsp40在肺肿瘤组织中的可能表达，并建立了一种酶联免疫吸附测定（ELISA）方法，以使用纯化的人Hsp40检测血清中针对Hsp40的IgG抗体。从130名正常受试者和50名肺癌患者中获取血清。肺肿瘤组织和细胞特异性过表达Hsp40，而在正常肺组织中未检测到这种表达。与正常血清相比，肺癌患者血清中针对Hsp40的自身抗体水平明显更高。本研究首次证明Hsp40在人肿瘤组织中的过表达以及血清中针对Hsp40的自身抗体的相关存在。这些结果表明，肿瘤细胞中Hsp40的过表达可能被识别为自身抗原。
