Joshi M, Dhar U
G.B. Pant Institute of Himalayan Environment and Development, Kosi-Katarmal, 263643 Almora, (UA), India.
Plant Cell Rep. 2003 Jun;21(10):933-9. doi: 10.1007/s00299-003-0601-1. Epub 2003 Apr 3.
This is the first report of a micropropagation protocol for Saussurea obvallata (DC.) Edgew. (Asteraceae), a rare, threatened and near-endemic medicinal herb of the Indian Himalayan region. Multiple shoots were formed from epicotyle explants on Murashige and Skoog (MS) medium supplemented with 1.0 microM kinetin and 0.25 microM alpha-naphthaleneacetic acid. A maximum of five shoots were obtained from one explant in a 75-day culture period. The effect of subsequent subcultures on shoot formation was also studied. After 100% in vitro rooting was obtained in half-strength MS supplemented with 2.5 microM indole-3-butyric acid, the plantlets were transferred to ex vitro conditions. Following a 15-day in vitro rooting period and 12 days of ex vitro acclimatization, 66.7% of the plantlets had established in the field. Application of this protocol has the potential to substantially reduce the pressure on natural populations.
这是关于绵头雪兔子（菊科）微繁殖体系的首次报道，绵头雪兔子是印度喜马拉雅地区一种珍稀、濒危且近乎地方特有的药用植物。在添加了1.0微摩尔激动素和0.25微摩尔α-萘乙酸的Murashige和Skoog（MS）培养基上，上胚轴外植体形成了多个芽。在75天的培养期内，一个外植体最多可获得5个芽。还研究了后续继代培养对芽形成的影响。在添加了2.5微摩尔吲哚-3-丁酸的1/2强度MS培养基中100%实现离体生根后，将幼苗转移到离体条件下。经过15天的离体生根期和12天的离体驯化，66.7%的幼苗在田间定植。应用该体系有可能大幅减轻对自然种群的压力。
