Makunga N P, Jäger A K, Van Staden J
Research Centre for Plant Growth and Development, School of Botany and Zoology, University of Natal Pietermaritzburg, Private Bag X01, 3209 Scottsville, South Africa.
Plant Cell Rep. 2003 Jun;21(10):967-73. doi: 10.1007/s00299-003-0623-8. Epub 2003 Apr 23.
The micropropagation of T. garganica, a medicinally important plant, was investigated as an option for conservation purposes as wild populations are becoming sparse. A Murashige and Skoog agar medium supplemented with 0.5 mg l(-1) alpha-naphthaleneacetic acid and 1.5 mg l(-1) benzyl-6-adenine significantly improved the production of multiple shoots directly from petiole and leaflet explants compared to other plant growth regulator (PGR) combinations. Medium free of PGRs promoted rooting at a low incidence. Acclimatisation was low--52% plantlet survival--as micropropagated plantlets were highly susceptible to fungal rot once removed from culture. Application of antifungal agents to in vitro-derived plantlets as pre- and post-acclimatisation treatments during transplanting significantly reduced ex vitro mortality.
药用植物加尔加诺百里香（Teucrium garganica）的微繁殖作为一种保护手段进行了研究，因为其野生种群正变得稀少。与其他植物生长调节剂（PGR）组合相比，添加了0.5毫克/升α-萘乙酸和1.5毫克/升苄基-6-腺嘌呤的Murashige和Skoog琼脂培养基显著提高了直接从叶柄和小叶外植体产生的丛生芽数量。不含PGR的培养基促进生根，但发生率较低。驯化率较低——植株存活率为52%——因为微繁殖的植株一旦从培养中移出，极易感染真菌腐烂。在移栽过程中，将抗真菌剂应用于离体培养的植株作为驯化前和驯化后的处理，显著降低了驯化后的死亡率。
