deS Senanayake P, Warner M R, Brosnihan K B, Levy M N, Ferrario C M
Cleveland Clinic Foundation, Department of Brain and Vascular Research, OH 44195.
Peptides. 1992 Nov-Dec;13(6):1165-73. doi: 10.1016/0196-9781(92)90024-w.
Neuropeptide Y-like immunoreactivity (NPY-LI) in dog plasma was characterized and quantified using three extraction methods (Sep-Pak:acetonitrile, HCl:ethanol, and ethanol). Sep-Pak extraction yields the best recovery and preserves the integrity of the peptide. Oxidized NPY is not generated during blood collection. Using two antisera of different specificities, at least three peptide forms in normal dog arterial and venous plasma were detected. A peptide with retention times similar to oxidized NPY or peptide YY is the major component of plasma NPY-LI under basal conditions, but NPY(1-36) predominates during sympathetic stimulation. The mature peptide in dog plasma is similar to human NPY. The antiserum ABII provides a more accurate measure of circulating NPY(1-36) and its oxidized form. The antiserum ABI is useful for detecting NPY-like fragments.
使用三种提取方法（Sep-Pak：乙腈、盐酸：乙醇和乙醇）对犬血浆中的神经肽Y样免疫反应性（NPY-LI）进行了表征和定量。Sep-Pak提取法回收率最高，并能保持肽的完整性。采血过程中不会产生氧化型NPY。使用两种不同特异性的抗血清，在正常犬动脉和静脉血浆中检测到至少三种肽形式。在基础条件下，保留时间与氧化型NPY或肽YY相似的一种肽是血浆NPY-LI的主要成分，但在交感神经刺激期间，NPY(1-36)占主导地位。犬血浆中的成熟肽与人类NPY相似。抗血清ABII能更准确地检测循环中的NPY(1-36)及其氧化形式。抗血清ABI可用于检测NPY样片段。
