Hegbrant J, Thysell H, Ekman R
Department of Nephrology, University Hospital, Lund, Sweden.
Peptides. 1995;16(3):395-7. doi: 10.1016/0196-9781(94)00206-l.
Increased plasma levels of neuropeptide Y (NPY)-like immunoreactivity (p-NPY-LI) have been described in hemodialysis (HD) patients. In this investigation the effect of a standard HD on p-NPY-LI, analyzed by radioimmunoassay and reverse-phase high performance liquid chromatography (HPLC), was studied. During dialysis p-NPY-LI increased from 128 +/- 5 to 154 +/- 8 pmol/l (p < 0.01). The change in p-NPY-LI during the treatment correlated with the ultrafiltration volume (rs = +0.72, p < 0.05). The HPLC separation revealed a complex pattern of NPY-immunoreactive peptides. This was true of the plasma of the control subjects as well as of the uremic plasma. In the case of the controls and the HD patients prior to dialysis, the amount of NPY-LI was rather small. After the dialysis qualitative as well as quantitative changes of the chromatogram were found. Some of the peaks seemed to have a retention time similar to that of the known fragments of synthetic human NPY used as markers. In conclusion, an increase in p-NPY-LI occurred during the dialysis, probably due to fluid removal. The increased level of NPY-LI in uremic plasma represents a mixture of different NPY fragments.
血液透析（HD）患者血浆中神经肽Y（NPY）样免疫反应性（p-NPY-LI）水平升高已有相关报道。在本研究中，采用放射免疫分析和反相高效液相色谱法（HPLC）研究了标准血液透析对p-NPY-LI的影响。透析过程中，p-NPY-LI从128±5皮摩尔/升升至154±8皮摩尔/升（p<0.01）。治疗期间p-NPY-LI的变化与超滤量相关（rs=+0.72，p<0.05）。HPLC分离显示NPY免疫反应性肽呈现复杂模式。对照受试者血浆和尿毒症血浆均如此。对于对照组和透析前的HD患者，NPY-LI的量相当少。透析后，色谱图出现了定性和定量变化。一些峰的保留时间似乎与用作标记的合成人NPY已知片段相似。总之，透析过程中p-NPY-LI升高，可能是由于液体清除所致。尿毒症血浆中升高的NPY-LI水平代表不同NPY片段的混合物。
