Obika S, Hemamayi R, Masuda T, Sugimoto T, Nakagawa S, Mayumi T, Imanishi T
Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamadaoka, Suita, Osaka 565-0871, Japan.
Nucleic Acids Res Suppl. 2001(1):145-6. doi: 10.1093/nass/1.1.145.
We have previously reported that 2'-O,4'-C-methylene bridged nucleic acid (2',4'-BNA) had unprecedented binding affinity towards its complementary RNA. We reported herein that the antisense 2',4'-BNA oligonucleotides showed potent inhibitory effect on gene expression of Intercellular Adhesion Molecule-1 (ICAM-1) in living cells. The contribution of RNase H to this antisense effect and adequate stability of 2',4'-BNA oligonucleotides to enzymatic degradation were also demonstrated.
我们之前曾报道，2'-O,4'-C-亚甲基桥连核酸（2',4'-BNA）对其互补RNA具有前所未有的结合亲和力。我们在此报道，反义2',4'-BNA寡核苷酸对活细胞中细胞间黏附分子-1（ICAM-1）的基因表达显示出强效抑制作用。还证实了RNase H对这种反义效应的作用以及2',4'-BNA寡核苷酸对酶促降解具有足够的稳定性。
