Aiken K D, Roth K A
Department of Pathology, Washington University School of Medicine, St Louis, Missouri 63110.
Dev Dyn. 1992 Aug;194(4):303-10. doi: 10.1002/aja.1001940406.
Precise spatial interrelationships exist between substance P, serotonin, and secretin containing enteroendocrine cells in the gastrointestinal tract of mice. In the proximal small intestine these products are coexpressed in various combinations in single enteroendocrine cells along the crypt to villus axis in a pattern that suggests the sequential expression of substance P, serotonin, and secretin. In this report we use bromodeoxyuridine (BrdU) and multilabeling immunohistochemistry to define the temporal and spatial interrelationships between substance P, serotonin, and secretin immunoreactive cells in the mouse proximal small intestine. Our findings demonstrate the sequential expression of substance P, serotonin, and secretin in a population of upwardly migrating enteroendocrine cells and, furthermore, identify a population of crypt associated cells coexpressing substance P and serotonin that fails to traverse this pathway. The lack of secretin immunoreactive cells in the crypts suggests that local factors present in the crypts and/or on villi regulate secretin expression. The combined use of BrdU and multilabeling immunohistochemistry provides a method for defining enteroendocrine cell differentiation pathways throughout the gastrointestinal tract.
在小鼠胃肠道中，P物质、血清素和含促胰液素的肠内分泌细胞之间存在精确的空间相互关系。在近端小肠中，这些产物以各种组合在单个肠内分泌细胞中沿隐窝到绒毛轴共同表达，其模式表明P物质、血清素和促胰液素是顺序表达的。在本报告中，我们使用溴脱氧尿苷（BrdU）和多重标记免疫组织化学来确定小鼠近端小肠中P物质、血清素和促胰液素免疫反应性细胞之间的时间和空间相互关系。我们的研究结果表明，P物质、血清素和促胰液素在一群向上迁移的肠内分泌细胞中顺序表达，此外，还鉴定出一群共同表达P物质和血清素但未能通过该途径的隐窝相关细胞。隐窝中缺乏促胰液素免疫反应性细胞表明，隐窝中和/或绒毛上存在的局部因子调节促胰液素的表达。BrdU和多重标记免疫组织化学的联合使用提供了一种确定整个胃肠道肠内分泌细胞分化途径的方法。
