Roth K A, Gordon J I
Department of Pathology, Washington University School of Medicine, Saint Louis, MO 63110.
Proc Natl Acad Sci U S A. 1990 Aug;87(16):6408-12. doi: 10.1073/pnas.87.16.6408.
The mammalian intestinal epithelium undergoes continuous and rapid renewal of its four principal terminally differentiated cell types. These cells arise from multipotent stem cells located at or near the base of the crypts of Lieberkühn. The differentiation process is precisely organized along two spatial dimensions (axes)--from the crypt to the villus tip and from the duodenum to the colon. The enteroendocrine cell population provides a sensitive marker of the intestine's topologic differentiation. At least 15 different regionally distributed subsets have been described based on their principal neuroendocrine products. We have used immunocytochemical methods to characterize the spatial relationships of the serotonin-, secretin-, and substance P-containing enteroendocrine cell subsets in normal adult C57BL/6J x LT/Sv mice as well as in transgenic littermates that contain rat liver fatty acid-binding protein-human growth hormone fusion genes. Our results reveal precise spatial interrelationships between these populations and suggest a differentiation pathway that may involve the sequential expression of substance P, serotonin, and secretin.
哺乳动物的肠上皮细胞的四种主要终末分化细胞类型会持续快速更新。这些细胞源自位于利伯库恩隐窝底部或其附近的多能干细胞。分化过程沿着两个空间维度（轴）精确组织——从隐窝到绒毛顶端，以及从十二指肠到结肠。肠内分泌细胞群体是肠道拓扑分化的一个敏感标志物。根据其主要神经内分泌产物，已描述了至少15种不同的区域分布亚群。我们使用免疫细胞化学方法来表征正常成年C57BL/6J×LT/Sv小鼠以及含有大鼠肝脏脂肪酸结合蛋白-人生长激素融合基因的转基因同窝小鼠中含5-羟色胺、促胰液素和P物质的肠内分泌细胞亚群的空间关系。我们的结果揭示了这些群体之间精确的空间相互关系，并提示了一条可能涉及P物质、5-羟色胺和促胰液素顺序表达的分化途径。
