Heazlewood Joshua L, Howell Katharine A, Millar A Harvey
Plant Molecular Biology Group, Biochemistry and Molecular Biology, School of Biomedical and Chemical Sciences, Faculty of Life and Physical Sciences, The University of Western Australia, 35 Stirling Hwy, Crawley 6009, Western Australia, Australia.
Biochim Biophys Acta. 2003 Jul 10;1604(3):159-69. doi: 10.1016/s0005-2728(03)00045-8.
The NADH:ubiquinone oxidoreductase of the mitochondrial respiratory chain is a large multisubunit complex in eukaryotes containing 30-40 different subunits. Analysis of this complex using blue-native gel electrophoresis coupled to tandem mass spectrometry (MS) has identified a series of 30 different proteins from the model dicot plant, Arabidopsis, and 24 different proteins from the model monocot plant, rice. These proteins have been linked back to genes from plant genome sequencing and comparison of this dataset made with predicted orthologs of complex I components in these plants. This analysis reveals that plants contain the series of 14 highly conserved complex I subunits found in other eukaryotic and related prokaryotic enzymes and a small set of 9 proteins widely found in eukaryotic complexes. A significant number of the proteins present in bovine complex I but absent from fungal complex I are also absent from plant complex I and are not encoded in plant genomes. A series of plant-specific nuclear-encoded complex I associated subunits were identified, including a series of ferripyochelin-binding protein-like subunits and a range of small proteins of unknown function. This represents a post-genomic and large-scale analysis of complex I composition in higher plants.
线粒体呼吸链中的NADH：泛醌氧化还原酶在真核生物中是一个大型多亚基复合体，包含30 - 40个不同的亚基。利用蓝色非变性凝胶电泳结合串联质谱（MS）对该复合体进行分析，已从模式双子叶植物拟南芥中鉴定出一系列30种不同的蛋白质，从模式单子叶植物水稻中鉴定出24种不同的蛋白质。这些蛋白质已与植物基因组测序中的基因相关联，并将该数据集与这些植物中复合体I成分的预测直系同源物进行了比较。该分析表明，植物含有在其他真核生物和相关原核生物酶中发现的一系列14个高度保守的复合体I亚基，以及在真核复合体中广泛存在的一小套9种蛋白质。牛复合体I中存在但真菌复合体I中不存在的大量蛋白质在植物复合体I中也不存在，并且在植物基因组中没有编码。鉴定出了一系列植物特异性的核编码复合体I相关亚基，包括一系列铁载体结合蛋白样亚基和一系列功能未知的小蛋白质。这代表了对高等植物复合体I组成的后基因组和大规模分析。
