Meyer Etienne H, Taylor Nicolas L, Millar A Harvey
ARC Center of Excellence in Plant Energy Biology, The University of Western Australia, 35 Stirling Highway, Crawley WA 6009, Australia.
J Proteome Res. 2008 Feb;7(2):786-94. doi: 10.1021/pr700595p. Epub 2008 Jan 12.
Analyzing highly hydrophobic proteins is a challenge for identification protocols based on gel separation and mass spectrometry. We combined Blue Native and 2D tricine gel electrophoresis to allow separation and identification of respiratory complex subunits from Arabidopsis mitochondria. We identified many of the highly hydrophobic mitochondrion-encoded subunits (GRAVY scores between +0.6 to +1.4) and also found a number of nucleus-encoded proteins associated with complex I for the first time in plants.
对于基于凝胶分离和质谱分析的鉴定方法而言，分析高度疏水的蛋白质是一项挑战。我们将蓝色非变性凝胶电泳和二维十二烷基肌氨酸钠凝胶电泳相结合，以实现对拟南芥线粒体呼吸复合体亚基的分离和鉴定。我们鉴定出了许多高度疏水的线粒体编码亚基（亲水性氨基酸平均偏差分数在+0.6至+1.4之间），并且首次在植物中发现了一些与复合体I相关的核编码蛋白。
