Glatz Frank R, Kalkanis James, Neumeister Michael, Suchy Hans, Lyons Sharon, Mowlavi Arian
Department of Otolaryngology-Head & Neck Surgery, Southern Illinois University School of Medicine, 301 North 8th Street, Springfield, IL, USA.
Laryngoscope. 2003 Jul;113(7):1113-7. doi: 10.1097/00005537-200307000-00003.
To compare the volume retention of injected preadipocytes with that of standard fat injection in a paralyzed rabbit true vocal cord.
Prospective analysis with blinded data collection.
Thirteen New Zealand white rabbits were divided into two groups. Group 1 consisted of seven animals undergoing left-side vocal cord paralysis by resection of a 1-cm segment of the left-side recurrent laryngeal nerve and abdominal fat harvest for isolation of preadipocytes. Preadipocytes were cultured under sterile conditions in cell culture media. Animals in group 2 also underwent left-side vocal cord paralysis without fat harvest. After 10 to 14 days, in a second procedure, group 1 underwent injection of 0.1 mL cultured autologous preadipocytes, and group 2 underwent routine injection of 0.1 mL abdominal fat harvested during the same procedure. At 6 and 12 months, volumetric analysis was performed.
Volume analysis at 6 months showed a mean volume of 0.029 mL retained fat in group 2 representing a retention of approximately 29% (SD = 0.023) of the original injected volume. Retention in group 1 animals approximated 0.002 mL (SD = 0.0024) or 2% of the injected volume. Analysis at 12 months showed a mean volume of 0.008 mL (SD = 0.0078) in group 2 and of 0.002 mL (SD = 0.0015) in group 1. Group 2 showed significantly higher volumes of the injected fat at 6 and 12 months (P <.033).
Volumes obtained with standard fat injection were superior to those obtained with preadipocyte injection at both 6 and 12 months.
比较在麻痹的兔真实声带中注射前脂肪细胞与标准脂肪注射后的体积保留情况。
前瞻性分析，数据收集采用盲法。
将13只新西兰白兔分为两组。第1组由7只动物组成，通过切除左侧喉返神经1厘米节段造成左侧声带麻痹，并采集腹部脂肪以分离前脂肪细胞。前脂肪细胞在无菌条件下于细胞培养基中培养。第2组动物也接受左侧声带麻痹，但不采集脂肪。10至14天后，在第二次手术中，第1组注射0.1毫升培养的自体前脂肪细胞，第2组注射在同一手术过程中采集的0.1毫升腹部脂肪。在6个月和12个月时进行体积分析。
6个月时的体积分析显示，第2组保留脂肪的平均体积为0.029毫升，约占原始注射体积的29%（标准差=0.023）。第1组动物的保留量约为0.002毫升（标准差=0.0024），即注射体积的2%。12个月时的分析显示，第2组平均体积为0.008毫升（标准差=0.0078），第1组为0.002毫升（标准差=0.0015）。第2组在6个月和12个月时注射脂肪的体积明显更高（P<.033）。
在6个月和12个月时，标准脂肪注射获得的体积均优于前脂肪细胞注射获得的体积。
