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[带蒂黏膜瓣移位术后声带固有层浅层组织学:犬实验研究]

[Vocal fold superficial layer of lamina propria histology after the position of mucosa pediculated flap: canine experimental study].

作者信息

Varela David Greco, Grellet Marcos

机构信息

Hospital Santa Izabel.

出版信息

Braz J Otorhinolaryngol. 2005 May-Jun;71(3):318-24. doi: 10.1016/s1808-8694(15)31329-x. Epub 2005 Dec 14.

Abstract

any techniques were applied to treat patients with sulcus vocalis and scarred vocal folds. Their results were not good enough. In the Technique of Vocal Fold Pediculated Mucosa Flap, an anterior pediculated flap of vocal fold is positioned on the superficial layer of the lamina propria, below the free margin. : To describe histological postoperative findings on the superficial layer of lamina propria during the application of the technique Vocal Fold Pediculated Mucosa Flap. The following parameters were compared between tested and control groups: total, type I and type III collagen and number of cellular nucleus. : experimental. : Fifteen dogs were used. One vocal fold was submitted to the intervention and the other was left as control. Each group of three dogs was sacrificed on 10, 30, 90, 180 and 360 days after the experimental surgery. Hematoxylin and eosin (H.E.) and Syrius Red were the staining techniques used. : Type I and total collagen suggested increased results in the tested group on postoperative days 90 and 180, nevertheless there was statistical significance only on postoperative day 180 (p<0.05). Type III collagen group area was less significant than the control group on postoperative day 180 (p<0.05). The number of cellular nucleus was increased on the 10th postoperative day, but decreased after the 30th day. : The findings about total and type I collagen and the amount of cellular nucleus on the superficial layer of lamina propria were similar to laryngeal postoperative studies in dogs. More complex studies would contribute with new data about the present subject.

摘要

应用了多种技术来治疗声带沟和声带瘢痕化患者。但他们的效果并不理想。在声带带蒂黏膜瓣技术中,声带的前带蒂瓣位于固有层浅层,游离缘下方。:描述在应用声带带蒂黏膜瓣技术过程中固有层浅层的术后组织学发现。比较试验组和对照组的以下参数:总胶原蛋白、I型和III型胶原蛋白以及细胞核数量。:实验性。:使用了15只狗。一侧声带接受干预,另一侧作为对照。每组三只狗在实验手术后的第10、30、90、180和360天处死。使用苏木精和伊红(H.E.)以及天狼星红染色技术。:I型和总胶原蛋白在术后第90天和180天试验组结果显示增加,不过仅在术后第180天有统计学意义(p<0.05)。III型胶原蛋白组面积在术后第180天比对照组差异不显著(p<0.05)。细胞核数量在术后第10天增加,但在第30天后减少。:关于固有层浅层总胶原蛋白和I型胶原蛋白以及细胞核数量的发现与犬类喉部术后研究相似。更复杂的研究将为该主题提供新的数据。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d07c/9450528/58117539e334/gr1.jpg

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