Menger M D, Pattenier J, Wolf B, Jäger S, Feifel G
Institute for Surgical Research, University of Munich, FRG.
Adv Exp Med Biol. 1992;317:799-803. doi: 10.1007/978-1-4615-3428-0_98.
Isolated pancreatic islets of Syrian golden hamsters were cryopreserved for 1 and 10 weeks, respectively. Following thawing islet mass was found more than 90%. Analysis of viability of these islets by dithizone staining revealed 94 +/- 4% (1 week cryopreservation) and 96 +/- 3% (10 weeks cryopreservation) positively stained islets. After implantation into dorsal skin-fold chambers of syngeneic animals, intravital fluorescence microscopy (5% FITC-dextran, i.v.) showed a regular microvascular network 10 days after implantation. The total diameter of the microvascular network was found similar in cryopreserved islets (day 10: 420.8 +/- 55.2 microns (1 week preservation) and 406.5 +/- 25.2 (10 weeks preservation)) as compared to non-preserved controls (day 10: 411.7 +/- 53.9 microns). Therefore we conclude that cryopreservation of hamster islet isografts for 1 and 10 weeks, respectively, does not alter viability as well as the potential for revascularization. Cryopreservation seems to be an adequate technique for long time storage prior to free transplantation.
将叙利亚金黄地鼠的分离胰岛分别冷冻保存1周和10周。解冻后发现胰岛质量超过90%。通过双硫腙染色分析这些胰岛的活力，结果显示阳性染色的胰岛分别为94±4%（冷冻保存1周）和96±3%（冷冻保存10周）。将其植入同基因动物的背部皮褶腔后，活体荧光显微镜检查（静脉注射5%异硫氰酸荧光素标记的葡聚糖）显示，植入10天后有规则的微血管网络。与未保存的对照组（第10天：411.7±53.9微米）相比，冷冻保存的胰岛微血管网络的总直径相似（第10天：420.8±55.2微米（保存1周）和406.5±25.2微米（保存10周））。因此，我们得出结论，将仓鼠胰岛同种异体移植物分别冷冻保存1周和10周，不会改变其活力以及血管再生的潜力。冷冻保存似乎是一种在自由移植前进行长期储存的合适技术。
