Ueno Takaaki, Kagawa Toshimasa, Fukunaga Jyoji, Mizukawa Nobuyoshi, Kanou Miwa, Fujii Takashi, Sugahara Toshio, Yamamoto Toshio
Departments of Oral and Maxillofacial Reconstructive Surgery, Okayama University Graduate School of Medicine and Dentistry, Japan.
Ann Plast Surg. 2003 Jul;51(1):77-83. doi: 10.1097/01.SAP.0000054180.78960.15.
Grafted periosteum has a rich potential to induce heterotopic bone formation. In the current study the authors investigate whether autogenous periosteal grafts can regenerate the mandibular head in a rabbit model. They removed the mandibular head of Japanese white rabbits and grafted tibial periosteum to the cut surface of the mandible. Grafted periosteum was observed histologically and radiographically at day 7, 14, 21, and 45 after surgery. At day 7 after grafting, grafted tissue showed remarkable cell proliferation. By 14 days these cells had differentiated into chondrocytes to form cartilage, and endochondral ossification took place after 21 days. At 45 days after surgery, soft X-ray findings showed a newly formed mandibular head, which was similar histologically to that of a normal mandibular head. The cut mandible without periosteal graft showed no regeneration. These findings indicate that grafted periosteum can regenerate the mandibular head without special procedures such as bone fixation in a rabbit model, and suggest that this technique may be useful clinically.
移植的骨膜具有诱导异位骨形成的巨大潜力。在本研究中，作者调查了自体骨膜移植能否在兔模型中使下颌头再生。他们切除了日本白兔的下颌头，并将胫骨骨膜移植到下颌骨的切面。在术后第7天、14天、21天和45天对移植的骨膜进行组织学和放射学观察。移植后第7天，移植组织显示出明显的细胞增殖。到14天时，这些细胞已分化为软骨细胞以形成软骨，21天后发生软骨内成骨。术后45天，软X线检查结果显示有新形成的下颌头，其组织学表现与正常下颌头相似。未进行骨膜移植的截断下颌骨未显示再生。这些发现表明，在兔模型中，移植的骨膜无需诸如骨固定等特殊程序即可使下颌头再生，并提示该技术可能在临床上有用。
