Marukawa K, Ueki K, Alam S, Shimada M, Nakagawa K, Yamamoto E
Department of Oral and Maxillofacial Surgery, Graduate School of Medicine, Kanazawa University, 13-1 Takaramachi, Kanazawa 920-8641, Japan.
Br J Oral Maxillofac Surg. 2006 Apr;44(2):141-5. doi: 10.1016/j.bjoms.2005.04.009. Epub 2005 Jun 22.
We examined the expression of bone morphogenetic protein-2 (BMP-2) and proliferating cell nuclear antigen (PCNA) during distraction osteogenesis in the mandible in rabbits. Twenty-four rabbits each had an osteotomy of the left mandibular body, and distraction devices were fixed. The bone was distracted at a rate of 1mm/day for 10 days. Four rabbits were killed at each of 1, 3, 7, 14, and 28 days after completion of distraction, and the mandibles examined radiographically, histologically, and immunohistochemically. Four rabbits that had not been operated on served as controls. Immunohistochemical analysis showed that BMP-2 and PCNA both appeared initially at the edge of the osteogenesis, but tended to disappear after 14 days. After 1, 3, 7, and 14 days after distraction, the ratio of stained cells was significantly higher than in the control group (p<0.05), during the period that active bone formation was shown radiographically and histologically. These results suggest that BMP-2 plays an important part in the induction of bone formation during distraction osteogenesis.
我们研究了兔下颌骨牵张成骨过程中骨形态发生蛋白-2（BMP-2）和增殖细胞核抗原（PCNA）的表达。24只兔均进行了左下颌骨体截骨术，并固定牵张装置。以每天1毫米的速度牵张骨组织，持续10天。在牵张完成后的第1、3、7、14和28天，分别处死4只兔，对下颌骨进行影像学、组织学和免疫组织化学检查。4只未做手术的兔作为对照。免疫组织化学分析显示，BMP-2和PCNA最初均出现在成骨边缘，但在14天后趋于消失。在牵张后的第1、3、7和14天，染色细胞比例显著高于对照组（p<0.05），此期间影像学和组织学显示有活跃的骨形成。这些结果表明，BMP-2在牵张成骨过程中对骨形成的诱导起重要作用。
