Franzoni M F, Canonaco M, Frova A, Tavolaro R
Dipartimento di Biologia Animale, Università di Torino, Italy.
Eur J Histochem. 1992;36(4):455-66.
The distribution of brain natriuretic peptide (BNP)-like immunoreactivity (ir) was studied in the brain of a urodele amphibian, the crested newt Triturus carnifex Laur. BNP-like immunoreactive neurons were found mainly in the caudal hypothalamus (retro- and supra-chiasmatic areas) and in the preoptic area. A widespread innervation throughout the brainstem as far as the spinal cord was also observed. By double immunostaining (after section incubation with a-BNP and a-tyrosine hydroxylase-TH-antibodies), close topographical relationships between BNP-like and TH-like immunoreactive neurons within the hypothalamus were found.
研究了有尾两栖动物冠欧螈（Triturus carnifex Laur.）脑中脑钠肽（BNP）样免疫反应性（ir）的分布。发现BNP样免疫反应性神经元主要位于下丘脑尾部（视交叉后和视交叉上区域）和视前区。还观察到其广泛支配整个脑干直至脊髓。通过双重免疫染色（切片先后用抗BNP和抗酪氨酸羟化酶-TH抗体孵育），发现下丘脑内BNP样和TH样免疫反应性神经元之间存在紧密的拓扑关系。
