Artero C, Mazzi V, Masucci A, Barale E, Franzoni M F
Dipartimento di Biologia Animale, Università degli Studi di Torino, Italy.
Eur J Histochem. 1995;39(3):183-94.
By using a histochemical procedure, the distribution of neurons containing dihydronicotinamide adenine dinucleotide diaphorase (NADPHd) was examined in the brain, retina and olfactory epithelium of the urodele amphibian Triturus carnifex. Positive nerve fibers and terminals were observed throughout the brain and cell bodies were seen within the telencephalon, optic tectum, brain stem, cerebellum and spinal cord. In the retina, NADPHd labeling was localized in the outer segment of photoreceptors and in some amacrine cells as well as in the outer and inner plexiform layers. In the olfactory epithelium, NADPHd labeling was found in the olfactory neurons. By comparing NADPHd distribution with nitric oxide synthase (NOS) immunoreactivity (using a polyclonal antiserum raised against mouse cerebellar NOS) it was found that NADPHd labeling and NOS immunoreaction patterns generally matched. The organization of NADPHd and NOS containing neurons in the central nervous system of the crested newt, which is simpler than in other vertebrates investigated, shows some peculiarities, such as the occurrence of NADPHD in the pinealocytes of the epiphysis, nucleus rubber of the brain stem and eminentia cerebellaris ventralis of the cerebellum.
通过组织化学方法，研究了有尾两栖动物真螈大脑、视网膜和嗅上皮中含二氢烟酰胺腺嘌呤二核苷酸黄递酶（NADPHd）的神经元分布。在整个大脑中均观察到阳性神经纤维和终末，在端脑、视顶盖、脑干、小脑和脊髓中可见细胞体。在视网膜中，NADPHd标记定位于光感受器的外段、一些无长突细胞以及外网状层和内网状层。在嗅上皮中，在嗅觉神经元中发现了NADPHd标记。通过将NADPHd分布与一氧化氮合酶（NOS）免疫反应性（使用针对小鼠小脑NOS产生的多克隆抗血清）进行比较，发现NADPHd标记和NOS免疫反应模式总体上相符。冠螈中枢神经系统中含NADPHd和NOS的神经元的组织比其他已研究的脊椎动物更为简单，显示出一些特点，例如松果体的松果体细胞、脑干的红核以及小脑的腹侧小脑隆起中存在NADPHD。
