Yücesoy Mine, Marol Serhat
Dokuz Eylül Universitesi Tip Fakültesi, Mikrobiyoloji ve Klinik Mikrobiyoloji Anabilim Dali, Izmir.
Mikrobiyol Bul. 2003 Jan;37(1):59-63.
This study was performed to determine the esterase activity of Candida species and to search the usefulness of tween 80 opacity test for the identification. Totally 124 Candida strains (66 C. albicans, 25 C. tropicalis, 14 C. parapsilosis, 10 C. glabrata, 3 C. krusei, 2 C. kefyr, 2 C. guilliermondii, 1 C. lusitaniae, 1 C. rugosa) isolated from various clinical specimens were included in this study. Tween 80 opacity test was applied to the strains and the plates were evaluated after incubation at 30 degrees C for ten days, by examining daily, for the presence of an opaque zone. As a result, 63 of (%95) of C. albicans, 23 of (93%) C. tropicalis, 8 of (57%) of C. parapsilosis and 1 (10%) of C. glabrata strains were found positive for esterase activity, whereas the other strains were found negative. It can be concluded that, tween 80 opacity test is a simple and economical method and may be useful as a supplementary test for the identification of Candida species, by detecting esterase activities which have arised on different days.
本研究旨在测定念珠菌属的酯酶活性，并探索吐温80不透明试验在鉴定中的实用性。本研究纳入了从各种临床标本中分离出的124株念珠菌（66株白色念珠菌、25株热带念珠菌、14株近平滑念珠菌、10株光滑念珠菌、3株克柔念珠菌、2株凯菲念珠菌、2株季也蒙念珠菌、1株葡萄牙念珠菌、1株皱落念珠菌）。对这些菌株进行吐温80不透明试验，并将平板在30℃孵育10天后进行评估，每天检查是否存在不透明区。结果显示，白色念珠菌中有63株（95%）、热带念珠菌中有23株（93%）、近平滑念珠菌中有8株（57%）、光滑念珠菌中有1株（10%）的菌株酯酶活性呈阳性，而其他菌株呈阴性。可以得出结论，吐温80不透明试验是一种简单且经济的方法，通过检测在不同天数出现的酯酶活性，可作为念珠菌属鉴定的补充试验。
