从尼泊尔分离出的念珠菌属的抗真菌药敏性及表型毒力标志物
Antifungal susceptibility and phenotypic virulence markers of Candida species isolated from Nepal.
作者信息
Subramanya Supram Hosuru, Baral Bharat Prasad, Sharan Nawal Kishor, Nayak Niranjan, Metok Yang, Sathian Brijesh, Bairy Indira, Gokhale Shishir
机构信息
Manipal College of Medical Sciences, Pokhara, Nepal.
Melaka Manipal Medical College, Manipal, India.
出版信息
BMC Res Notes. 2017 Nov 2;10(1):543. doi: 10.1186/s13104-017-2852-x.
OBJECTIVE
Candida species are part of the commensal microflora in many anatomical sites of the human body; however, breach in the integrity of the body part and impaired immunity of the host can lead to invasive candidiasis. A number of virulence determinants could contribute towards its pathogenicity. Thus we attempted to evaluate the in vitro expression of different virulence factors among clinical isolates of Candida species and assayed their susceptibility patterns against a range of antifungal agents.
RESULT
Of the total of 71 isolates we obtained, 48 (67.6%) were Candida albicans, 11 (15.49%) Candida tropicalis, 09 (12.67%) Candida glabrata and 03 (4.22%) were Candida krusei. Proteinase, phospholipase and esterase production could be revealed amongst 43 (60.56%), 44 (61.97%) and 49 (69.01%) isolates respectively. None of the isolates showed DNAase activity. Fifty-five (77.39%) isolates were biofilm producers, and 53 (74.6%) exhibited high cell surface hydrophobicity.
目的
念珠菌属是人体许多解剖部位共生微生物群的一部分；然而，身体部位完整性的破坏和宿主免疫力的受损可导致侵袭性念珠菌病。许多毒力决定因素可能导致其致病性。因此，我们试图评估念珠菌属临床分离株中不同毒力因子的体外表达情况，并测定它们对一系列抗真菌药物的敏感性模式。
结果
在我们获得的71株分离株中，48株（67.6%）为白色念珠菌，11株（15.49%）为热带念珠菌，9株（12.67%）为光滑念珠菌，3株（4.22%）为克柔念珠菌。分别在43株（60.56%）、44株（61.97%）和49株（69.01%）分离株中检测到蛋白酶、磷脂酶和酯酶的产生。所有分离株均未显示DNA酶活性。55株（77.39%）分离株可产生生物膜，53株（74.6%）表现出高细胞表面疏水性。
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