Volozhin A I, Bondarenko O A, Desiatnichenko K S, Kurdiumov S G, Lebedev V G
Patol Fiziol Eksp Ter. 2003 Apr-Jun(2):21-3.
The experimental study of the properties of the collagen- and hydroxyapatite-containing osteoplastic material HAPKOL-I by bone marrow hemopoiesis was made in comparison with existing material HAPKOL. HAPKOL-I is a working name of the material consisting of HAPKOL and composition of non-collagen bone proteins produced from xenogenic bone material. Long-term cultures of bone marrow from mice (C57B1/6xCBA)F1 of dexter type were used. Cultivation time was 7, 14, 21 and 28 days. Intensity of hemopoietic processes was assessed on the samples; absolute count of stromal and hemopoietic cells on the membrane was calculated. It was found that in long-term cultures of the bone marrow stromal and hemopoietic cells develop on the materials HAPKOL and HAPKOL-I, processes of formation of hemopoietic microenvironment provide normal hemopoiesis. On both materials there was a gradual increase of cell counts, a rise in the number of stromal and a fall in the number of hemopoietic cells. More intensive hemopoiesis on the material containing non-collagen bone proteins may be due to this factor.
通过骨髓造血对含胶原蛋白和羟基磷灰石的骨塑性材料HAPKOL-I的特性进行了实验研究，并与现有材料HAPKOL进行了比较。HAPKOL-I是由HAPKOL和从异种骨材料中产生的非胶原蛋白骨蛋白组成的材料的工作名称。使用来自（C57B1/6xCBA）F1品系的小鼠的长期骨髓培养物。培养时间为7、14、21和28天。评估样本上造血过程的强度；计算膜上基质细胞和造血细胞的绝对计数。结果发现，在骨髓基质细胞和造血细胞在材料HAPKOL和HAPKOL-I上的长期培养中，造血微环境的形成过程可提供正常造血。在这两种材料上，细胞计数都逐渐增加，基质细胞数量上升，造血细胞数量下降。含非胶原蛋白骨蛋白的材料上造血更活跃可能归因于这一因素。
