大肠杆菌tRNA识别元件的体外研究。
In vitro study of E. coli tRNA identity elements.
作者信息
Tamura T, Asahara H, Nameki N, Himeno H, Hasegawa T, Shimizu M
机构信息
Institute of Space and Astronautical Science, Kanagawa, Japan.
出版信息
Nucleic Acids Symp Ser. 1992(27):143-4.
Various tRNA transcripts were constructed to study the identity elements of E. coli tRNAs (Arg, Lys, Ala, Trp, Thr, Gly, Ser, Asn, Cys, His). Anticodon are involved in the identity elements in these tRNA species except the case of tRNA(Ala) and tRNA(Ser). Especially, the second and third positions of the anticodon are the recognition sites of E. coli tRNA(Arg), tRNA(Lys) and tRNA(Thr) for their cognate aminoacyl-tRNA synthetases. Discriminator base is an identity determinant of the above examined tRNAs except tRNA(Thr) and tRNA(Ser). In some cases, acceptor stem (Thr, Gly, His) and variable pocket (Arg, Ala) are considered to be the recognition elements.
构建了各种tRNA转录本以研究大肠杆菌tRNA（精氨酸、赖氨酸、丙氨酸、色氨酸、苏氨酸、甘氨酸、丝氨酸、天冬酰胺、半胱氨酸、组氨酸）的识别元件。除了tRNA（丙氨酸）和tRNA（丝氨酸）的情况外，反密码子参与了这些tRNA种类的识别元件。特别是，反密码子的第二位和第三位是大肠杆菌tRNA（精氨酸）、tRNA（赖氨酸）和tRNA（苏氨酸）与其同源氨酰-tRNA合成酶的识别位点。除了tRNA（苏氨酸）和tRNA（丝氨酸）外，鉴别碱基是上述所研究tRNA的识别决定因素。在某些情况下，接受茎（苏氨酸、甘氨酸、组氨酸）和可变环（精氨酸、丙氨酸）被认为是识别元件。
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