Urata H, Ueda Y, Usami Y, Akagi M
Osaka University of Pharmaceutical Sciences, Japan.
Nucleic Acids Symp Ser. 1992(27):3-4.
The hexadeoxynucleotide analog, L-d(CGCGCG) composed of L-deoxyribose was synthesized and clearly shown to have the same conformation and dynamic properties with natural D-d(CGCGCG) except for chirality with CD spectra. This unnatural hexanucleotide was not cleaved by bleomycin, an antitumor DNA cleaving drug, but was able to bind to the DNA binding domain of bleomycin to a similar extent with the natural one. These results strongly suggest the importance of the other moiety than the DNA binding domain for the specific DNA recognition of bleomycin. Thus, L-oligonucleotides are useful for the study of DNA-drug interactions.
由L-脱氧核糖组成的十六脱氧核苷酸类似物L-d(CGCGCG)被合成出来，并且通过圆二色光谱清楚地表明，除了手性之外，它与天然的D-d(CGCGCG)具有相同的构象和动力学性质。这种非天然的六核苷酸不会被抗肿瘤DNA切割药物博来霉素切割，但能够与博来霉素的DNA结合结构域以与天然六核苷酸相似的程度结合。这些结果有力地表明，对于博来霉素特异性识别DNA而言，除DNA结合结构域之外的其他部分也很重要。因此，L-寡核苷酸对于研究DNA-药物相互作用很有用。
