Johard H A, Lundquist C T, Rökaeus A, Nässel D R
Department of Zoology, Stockholm University, Sweden.
Regul Pept. 1992 Dec 11;42(3):123-34. doi: 10.1016/0167-0115(92)90092-9.
The localization of porcine galanin (pGAL) binding sites in the brain of the blowfly Phormia terraenovae was investigated by autoradiography using the following radioiodinated ligands: pGAL 1-29 (two isoforms), pGAL 15-29 and rat (r) GAL 1-29. The different porcine radioligands bound specifically with the following intensity: 125I-[Tyr26]-pGAL15-29 > > 125I-[Tyr26]-pGAL1-29 > > 125I-[Tyr9]-pGAL1-29. With rat galanin 125I-[Tyr9]-rGAL1-29 no specific binding could be shown. In addition, displacement of 125I-[Tyr26]-pGAL1-29 was tested with pGAL 1-29, pGAL 1-22 and pGAL 15-29 (at 0.1 nM-1 microM). A gradual displacement was achieved with increasing concentrations of pGAL 1-29 and pGAL15-29, whereas no displacement with pGAL 1-22 was detected. The results indicate that the C-terminal portion of pGAL is important for binding in the blowfly. The pGAL binding sites were localized in synaptic neuropils of the central body, the antennal lobes, the optic lobes, the pars intercerebralis and the subesophageal ganglion, all of which contain GAL-like immunoreactive neural processes.
利用放射自显影技术，使用以下放射性碘化配体，研究了猪甘丙肽（pGAL）结合位点在新陆原伏蝇大脑中的定位：pGAL 1 - 29（两种异构体）、pGAL 15 - 29和大鼠（r）GAL 1 - 29。不同的猪放射性配体以以下强度特异性结合：125I - [Tyr26] - pGAL15 - 29 >> 125I - [Tyr26] - pGAL1 - 29 >> 125I - [Tyr9] - pGAL1 - 29。对于大鼠甘丙肽125I - [Tyr9] - rGAL1 - 29，未显示出特异性结合。此外，用pGAL 1 - 29、pGAL 1 - 22和pGAL 15 - 29（浓度为0.1 nM - 1 microM）测试了125I - [Tyr26] - pGAL1 - 29的置换情况。随着pGAL 1 - 29和pGAL15 - 29浓度的增加，实现了逐渐置换，而未检测到pGAL 1 - 22的置换。结果表明，pGAL的C末端部分对于在新陆原伏蝇中的结合很重要。pGAL结合位点定位于中央体、触角叶、视叶、脑间部和咽下神经节的突触神经纤维网中，所有这些部位都含有甘丙肽样免疫反应性神经突起。
