Lundquist C T, Johard H A, Rökaeus A, Nässel D R
Department of Zoology, Stockholm University, Sweden.
Acta Biol Hung. 1993;44(1):51-4.
We have used immunocytochemical, immunochemical (RIA) and chromatographic methods (HPLC, gel filtration) to provide evidence for presence of GAL-like peptide(s) in the blowfly Phormia terraenovae. HPLC indicates the presence of several forms of GAL-like peptides. Immunocytochemistry showed that there are about 160 GAL-IR neurons in the brain and subesophageal ganglia supplying the central body, superior protocerebrum, the optic lobe and tritocerebral neuropil. Autoradiography of binding with 125I-labelled porcine GAL on brain sections revealed GAL binding sites in the central body complex and deutocerebrum. The presence of galanin-like peptide(s) and putative receptor sites in the fly brain suggest a role in neuromodulation in specific circuits.
我们已使用免疫细胞化学、免疫化学（放射免疫分析）和色谱方法（高效液相色谱、凝胶过滤）来证明新陆原伏蝇体内存在类甘丙肽。高效液相色谱表明存在几种形式的类甘丙肽。免疫细胞化学显示，在脑和咽下神经节中有大约160个向中央体、前脑上部、视叶和后脑神经纤维网供应的甘丙肽免疫反应性神经元。用125I标记的猪甘丙肽对脑切片进行结合放射自显影，结果显示在中央体复合体和中脑中有甘丙肽结合位点。果蝇脑中类甘丙肽和假定受体位点的存在表明其在特定神经回路的神经调节中发挥作用。
