Kirkpatrick F H, Woods G M, La Celle P L
Blood. 1975 Dec;46(6):945-54.
The stimulation by calcium and magnesium of ATPase activity of isolated ghosts, of water-soluble protein (spectrin), and of residual vesicles, derived from normal erythrocytes and from hereditary spherocytes (H.S.), has been measured. The ATPase activity found in normal water-soluble protein (WSP) at low levels of calcium (0.1-2.0 mM) is essentially absent in H.S. water-soluble protein, but the ATPase activity with magnesium and with high levels of calcium (60-100 mM) is the same in H.S. and normal WSP. Compared to normal, H.S. ghosts have increased Mg2+-stimulated activity. This increased activity is retained by the sedimentable vesicles ("residue") after extraction of the ghosts with 0.025 mM EDTA. The Ca2+, Mg2+-ATPase associated with the calcium pump is not significantly different in H.S.
已测定钙和镁对从正常红细胞和遗传性球形红细胞（H.S.）中分离出的血影、水溶性蛋白（血影蛋白）和残余囊泡的ATP酶活性的刺激作用。在低钙水平（0.1 - 2.0 mM）下正常水溶性蛋白（WSP）中发现的ATP酶活性在H.S.水溶性蛋白中基本不存在，但H.S.和正常WSP中镁以及高钙水平（60 - 100 mM）下的ATP酶活性相同。与正常情况相比，H.S.血影具有增强的Mg2 +刺激活性。在用0.025 mM EDTA提取血影后，这种增强的活性被可沉淀囊泡（“残余物”）保留。与钙泵相关的Ca2 +，Mg2 + - ATP酶在H.S.中没有显著差异。
