先天性球形红细胞增多症中红细胞膜的钙转运及三磷酸腺苷酶活性

Calcium transport and adenosine triphosphatase activities of erythrocyte membranes in congenital spherocytosis.

作者信息

Johnsson R, Santaholma S, Saris N E

出版信息

Scand J Clin Lab Invest. 1978 Apr;38(2):121-5. doi: 10.1080/00365517809156079.

Abstract

Calcium transport from red cells was measured in seventeen patients with congenital or hereditary spherocytosis (HS). The efflux remained at a lower level in resealed ghost cells of patients than in normal cells both in the presence and absence of adenosine triphosphate (ATP). We studied the activities of Ca2+,Mg2+-ATPase, ouabain-sensitive Na+,K+-ATPase, Mg2+-ATPase and Ca2+-(spectrin-)ATPase in cell membranes prepared by washing the cells with hypotonic medium. The mean +/-SD Ca2+,Mg2+-ATPase/Mg2+-ATPase of HS patients was 3.34 +/- 1.06, and 2.81 +/- 0.42 in control subjects. Na+,K+-ATPase/Mg2+-ATPase was 2.38 +/- 0.38 in HS cells compared to 2.01 +/- 0.41 in normal cells. Ca2+-ATPase/Mg2+-ATPase of HS membranes was 0.57 +/- 0.18 and the control value 0.43 +/- 0.08. These data indicate calcium retention in the erythrocytes of HS patients in spite of increases in Ca2+,Mg2+-ATPase activity in the majority of patients.

摘要

对17例先天性或遗传性球形红细胞增多症（HS）患者的红细胞钙转运进行了测定。无论有无三磷酸腺苷（ATP），患者重封血影细胞中的钙外流水平均低于正常细胞。我们研究了用低渗介质洗涤细胞制备的细胞膜中Ca2 +、Mg2 + -ATP酶、哇巴因敏感的Na +、K + -ATP酶、Mg2 + -ATP酶和Ca2 + -（血影蛋白）-ATP酶的活性。HS患者的平均±标准差Ca2 +、Mg2 + -ATP酶/Mg2 + -ATP酶为3.34±1.06，对照组为2.81±0.42。HS细胞中的Na +、K + -ATP酶/Mg2 + -ATP酶为2.38±0.38，正常细胞为2.01±0.41。HS细胞膜的Ca2 + -ATP酶/Mg2 + -ATP酶为0.57±0.18，对照值为0.43±0.08。这些数据表明，尽管大多数患者的Ca2 +、Mg2 + -ATP酶活性增加，但HS患者红细胞中的钙潴留情况仍存在。