Jin H
General Hospital of PLA, Beijing.
Zhonghua Bing Li Xue Za Zhi. 1992 Oct;21(5):278-80.
Two hundred male mice, weighing 18-24g, were randomized into four groups. A model of lung injury was established in mice by intratracheal administration of a single dose of domestic pingyangmycinum (Bleomycin A5). The dose-effect relationship for different doses of pingyangmycinum (0.05, 0.1, 0.2 mg) on the lung injury, and the change of hydroxyprolin (Hyp), ceruloplasmin (CP), angiotensin converting enzyme (ACE), superoxide dismutase (SOD) in mice were investigated. The results showed that the pathological changes of alveolitis and pulmonary interstitial fibrosis were more severe, and mortality of the mice was increased when pingyangmycinum dose was increased. During 2-6 weeks of this experiment, Hyp, CP, ACE and SOD in serum and Hyp in lung tissue were increased in different degrees. However, the activity of SOD in lung tissue was decreased. The possible mechanism of this lung injury is also discussed.
将200只体重为18 - 24克的雄性小鼠随机分为四组。通过气管内单次给予国产平阳霉素（博来霉素A5）建立小鼠肺损伤模型。研究了不同剂量（0.05、0.1、0.2毫克）平阳霉素对肺损伤的剂量 - 效应关系，以及小鼠体内羟脯氨酸（Hyp）、铜蓝蛋白（CP）、血管紧张素转换酶（ACE）、超氧化物歧化酶（SOD）的变化。结果显示，随着平阳霉素剂量增加，肺泡炎和肺间质纤维化的病理变化更严重，小鼠死亡率增加。在本实验的2 - 6周期间，血清中的Hyp、CP、ACE和SOD以及肺组织中的Hyp均有不同程度升高。然而，肺组织中SOD的活性降低。还讨论了这种肺损伤的可能机制。
