Wang Shao-Jie, Bai Wen, Wang Chun-Ling, Dai Zhong
Department of Traditional Chinese Medicine in People's Hospital of Peking University, Beijing 100044, China.
Zhongguo Zhong Yao Za Zhi. 2007 Dec;32(24):2623-7.
To study the prevention & treatment effect of cordyceps sinensi on bleomycin-induced pulmonary fibrosis (PF) in mice.
Rats were divided into 4 groups. In BM group (model), pulmonary interstitial fibrosis model was established through nasal dripping of 5 mg x kg(-1) bleomycin. In PCT group (prevention & treatment with cordyceps sinensi), cordyceps sinensi was orally administered 48 h before bleomycin dripping. In CT group (treatment with cordyceps sinensi), cordyceps sinensi was orally administered at 14 d after bleomycin dripping, and NC group (normal mice, control). At 28 d, bronchoalveolar lavage fluid (BALF) and lung tissue were collected, right lung received HE staining and masson staining, the change in cell micro-structure was observed under electron microscope, the hydroxyproline (HYP) content in lung tissue of left lung was detected through acidolysis method, and TGF-beta1, IFN-gamma and IL-4 concentration in BALF was detected through ELISA.
Compared with those in BM group, mouse lung coefficient decreased significantly (P < 0.05), alveolitis and fibrosis degree improved significantly in PCT and CT groups (P < 0.05), HYP content in lung decreased (P < 0.05). TGF-beta1 and 1L-4 content in BALF decreased significantly in each drug intervention group, IFN-gamma content increased significantly (P < 0.05).
The cordyceps sinensi can alleviate bleomycin-induced alveolitis and fibrosis degree in a certain degree. The mechanism of the alleviation is possibly due to its inhibition of TGF-beta1 expression and its regulation of imbalance of the type I and II cytokine in lung tissue.
研究冬虫夏草对博来霉素诱导的小鼠肺纤维化（PF）的防治作用。
将大鼠分为4组。BM组（模型组）通过滴鼻5mg·kg⁻¹博来霉素建立肺间质纤维化模型。PCT组（冬虫夏草防治组）在滴注博来霉素前48小时口服冬虫夏草。CT组（冬虫夏草治疗组）在滴注博来霉素后14天口服冬虫夏草，NC组为正常小鼠（对照组）。在第28天，收集支气管肺泡灌洗液（BALF）和肺组织，右肺进行苏木精-伊红（HE）染色和马松染色，在电子显微镜下观察细胞微观结构的变化，通过酸解法检测左肺肺组织中羟脯氨酸（HYP）含量，并通过酶联免疫吸附测定（ELISA）检测BALF中转化生长因子-β1（TGF-β1）、干扰素-γ（IFN-γ）和白细胞介素-4（IL-4）浓度。
与BM组相比，小鼠肺系数显著降低（P<0.05），PCT组和CT组的肺泡炎和纤维化程度显著改善（P<0.05），肺组织中HYP含量降低（P<0.05）。各药物干预组BALF中TGF-β1和IL-4含量显著降低，IFN-γ含量显著升高（P<0.05）。
冬虫夏草可在一定程度上减轻博来霉素诱导的肺泡炎和纤维化程度。减轻的机制可能是其抑制TGF-β¹表达并调节肺组织中I型和II型细胞因子的失衡。
