DePaola Angelo, Ulaszek Jodie, Kaysner Charles A, Tenge Bradley J, Nordstrom Jessica L, Wells Joy, Puhr Nancy, Gendel Steven M
Gulf Coast Seafood Laboratory, U.S. Food and Drug Administration, Dauphin Island, AL 36528, USA.
Appl Environ Microbiol. 2003 Jul;69(7):3999-4005. doi: 10.1128/AEM.69.7.3999-4005.2003.
Potential virulence attributes, serotypes, and ribotypes were determined for 178 pathogenic Vibrio parahaemolyticus isolates from clinical, environmental, and food sources on the Pacific, Atlantic, and Gulf Coasts of the United States and from clinical sources in Asia. The food and environmental isolates were generally from oysters, and they were defined as being pathogenic by using DNA probes to detect the presence of the thermostable direct hemolysin (tdh) gene. The clinical isolates from the United States were generally associated with oyster consumption, and most were obtained from outbreaks in Washington, Texas, and New York. Multiplex PCR was used to confirm the species identification and the presence of tdh and to test for the tdh-related hemolysin trh. Most of the environmental, food, and clinical isolates from the United States were positive for tdh, trh, and urease production. Outbreak-associated isolates from Texas, New York, and Asia were predominantly serotype O3:K6 and possessed only tdh. A total of 27 serotypes and 28 ribogroups were identified among the isolates, but the patterns of strain distribution differed between the serotypes and ribogroups. All but one of the O3:K6 isolates from Texas were in a different ribogroup from the O3:K6 isolates from New York or Asia. The O3:K6 serotype was not detected in any of the environmental and food isolates from the United States, and none of the food or environmental isolates belonged to any of the three ribogroups that contained all of the O3:K6 and related clinical isolates. The combination of serotyping and ribotyping showed that the Pacific Coast V. parahaemolyticus population appeared to be distinct from that of either the Atlantic Coast or Gulf Coast. The fact that certain serotypes and ribotypes contained both clinical and environmental isolates while many others contained only environmental isolates implies that certain serotypes or ribotypes are more relevant for human disease.
对来自美国太平洋、大西洋和墨西哥湾沿岸临床、环境及食品来源以及亚洲临床来源的178株致病性副溶血性弧菌分离株，测定了其潜在毒力属性、血清型和核糖型。食品和环境分离株一般来自牡蛎，通过使用DNA探针检测耐热直接溶血素（tdh）基因的存在来确定其为致病性。美国的临床分离株一般与食用牡蛎有关，大多数分离株来自华盛顿、得克萨斯州和纽约的疫情。采用多重PCR确认菌种鉴定及tdh的存在，并检测与tdh相关的溶血素trh。美国大多数环境、食品和临床分离株tdh、trh和脲酶产生呈阳性。来自得克萨斯州、纽约和亚洲与疫情相关的分离株主要为O3:K6血清型，且仅携带tdh。在分离株中总共鉴定出27种血清型和28个核糖组，但血清型和核糖组之间的菌株分布模式有所不同。得克萨斯州的O3:K6分离株中，除一株外，其余均与来自纽约或亚洲的O3:K6分离株属于不同的核糖组。在美国的任何环境和食品分离株中均未检测到O3:K6血清型，且食品或环境分离株均不属于包含所有O3:K6及相关临床分离株的三个核糖组中的任何一个。血清分型和核糖分型相结合表明，太平洋沿岸的副溶血性弧菌群体似乎与大西洋沿岸或墨西哥湾沿岸的群体不同。某些血清型和核糖型同时包含临床和环境分离株，而许多其他血清型和核糖型仅包含环境分离株，这一事实表明某些血清型或核糖型与人类疾病的相关性更强。
