Elprana D, Kuijpers W, Wessels J M, Wagener D J, van den Broek P
Department of Otorhinolarynology, University Hospital of Nijmegen, The Netherlands.
Anticancer Res. 1992 Nov-Dec;12(6B):2229-39.
Eight squamous cell carcinomas from the head and neck region were established as xenograft lines in nude mice and tested for their sensitivity to the antineoplastic drugs bleomycin and cisplatin. Tumor volume, histology, DNA flow cytometry and mitotic activity were used as parameters. One out of the 8 tumours appeared to be highly sensitive to bleomycin, while three other tumours were sensitive to both bleomycin and cisplatin. These observations are in good correlation with the reported data in patients. All chemosensitive tumours showed regrowth after the cytotoxic drug treatment had been completed. No change was seen in the chemosensitivity of other features of the regrown tumours, not even after repeated exposure to the drugs. Comparison of the tumour volume with the other parameters applied indicated that the tumour volume of squamous cell carcinomas was not always a reliable parameter for testing chemosensitivity, because of the important contribution of keratin to the tumour volume. It is concluded that additional parameters such as histological examination, DNA flow cytometry or mitotic activity are necessary in order to draw reliable conclusion on xenografts with a large avital component. In addition, DNA flow cytometry has proved to be very useful for the rapid screening of drug sensitivity.
将来自头颈部区域的8例鳞状细胞癌在裸鼠中建立异种移植系，并检测它们对抗肿瘤药物博来霉素和顺铂的敏感性。肿瘤体积、组织学、DNA流式细胞术和有丝分裂活性用作参数。8个肿瘤中有1个似乎对博来霉素高度敏感，而另外3个肿瘤对博来霉素和顺铂均敏感。这些观察结果与患者的报告数据高度相关。所有化疗敏感的肿瘤在细胞毒性药物治疗完成后均出现再生长。再生长肿瘤的其他特征的化疗敏感性未见变化，即使在反复接触药物后也是如此。将肿瘤体积与所应用的其他参数进行比较表明，由于角蛋白对肿瘤体积的重要贡献，鳞状细胞癌的肿瘤体积并不总是检测化疗敏感性的可靠参数。得出的结论是，为了对具有大量无活力成分的异种移植得出可靠结论，需要诸如组织学检查、DNA流式细胞术或有丝分裂活性等额外参数。此外，DNA流式细胞术已被证明对快速筛选药物敏感性非常有用。
