Tabakov V Iu, Tokmakova I L, Sezonov G V
Antibiot Khimioter. 1992 Oct;37(10):10-5.
Streptomyces hygroscopicus ATCC21705 is an organism producing bialaphos, a promising ecologically safe antibiotic of peptide nature. An unknown determinant was cloned within the 1.4-kb SsH fragment of chromosomal DNA of S. hygroscopicus. Its presence along with the bar gene in the hybrid plasmid PVGB21 increased expression of the phenotype resistance to bialaphos in the recipient strain of S. lividans TK 64. Plasmid PVG B22 carrying the isolated SsH fragment did not provide resistance to the antibiotic. Transcription from the cloned fragment was revealed by RNA-DNA hybridization. It was shown that the cloned determinant had a positive action on bar gene transcription correlating with a significant increase in the activity of N-acetyltransferase in the mycelium of the transformant pVG B21.
吸水链霉菌ATCC21705是一种产生双丙氨膦的生物体，双丙氨膦是一种有前景的具有肽性质的生态安全抗生素。在吸水链霉菌染色体DNA的1.4 kb SsH片段中克隆到一个未知决定簇。它与bar基因一起存在于杂交质粒PVGB21中，增加了变铅青链霉菌TK 64受体菌株对双丙氨膦的表型抗性表达。携带分离出的SsH片段的质粒PVG B22并未赋予对抗生素的抗性。通过RNA-DNA杂交揭示了来自克隆片段的转录。结果表明，克隆的决定簇对bar基因转录有正向作用，这与转化体pVG B21菌丝体中N-乙酰转移酶活性的显著增加相关。
