Avalos J, Geever R F, Case M E
Department of Genetics, University of Georgia, Athens 30602.
Curr Genet. 1989 Dec;16(5-6):369-72. doi: 10.1007/BF00340716.
Conidia of Neurospora crassa are sensitive to the herbicide bialaphos at concentrations of 160 mg/l in Westergaard's or Fries' minimal media. Plasmid pJA4 was constructed by inserting a truncated bar gene from Streptomyces hygroscopicus fused to the his-3 promoter from N. crassa into pUC19. The bar gene in plasmid pJA4 confers resistance to bialaphos when transformants are selected on a medium containing bialaphos. The bar gene can be used as an additional dominant selectable marker for transformation of fungi.
粗糙脉孢菌的分生孢子在韦斯特加德或弗里斯基本培养基中，对浓度为160毫克/升的除草剂双丙氨膦敏感。质粒pJA4是通过将来自吸水链霉菌的截短bar基因与粗糙脉孢菌的his-3启动子融合后插入pUC19构建而成。当在含有双丙氨膦的培养基上选择转化体时，质粒pJA4中的bar基因赋予对双丙氨膦的抗性。bar基因可用作真菌转化的另一个显性选择标记。
