Ang Eugenius S B C, Haydar Tarik F, Gluncic Vicko, Rakic Pasko
Department of Neurobiology, Yale Medical School, New Haven, Connecticut 06510, USA.
J Neurosci. 2003 Jul 2;23(13):5805-15. doi: 10.1523/JNEUROSCI.23-13-05805.2003.
We have used time-lapse multiphoton microscopy to map the migration and settling pattern of GABAergic interneurons that originate in the ganglionic eminence of the ventral forebrain and incorporate into the neocortex of the cerebral hemispheres. Imaging of the surface of the cerebral hemispheres in both explant cultures and brains of living mouse embryos revealed that GABAergic interneurons migrating within the marginal zone originate from three different sources and migrate via distinct and independent streams. After reaching their areal destination, interneurons descend into the underlying cortex to assume positions with isochronically generated, radially derived neurons. The dynamics and pattern of cell migration in the marginal zone (see movies, available at www.jneurosci.org) suggest that the three populations of interneurons respond selectively to distinct local cues for directing their migration to the appropriate areas and layers of the neocortex. This approach opens a new avenue for study of normal and abnormal neuronal migration in their native environment and indicate that interneurons have specific programs for their areal and laminar deployment.
我们利用延时多光子显微镜绘制了γ-氨基丁酸（GABA）能中间神经元的迁移和定居模式，这些中间神经元起源于腹侧前脑的神经节隆起，并整合到大脑半球的新皮质中。对离体培养物和活小鼠胚胎大脑中大脑半球表面的成像显示，在边缘区内迁移的GABA能中间神经元起源于三个不同的来源，并通过不同且独立的路径迁移。到达其区域目的地后，中间神经元下降到下层皮质，与同期产生的、径向衍生的神经元占据相同位置。边缘区内细胞迁移的动力学和模式（见电影，可在www.jneurosci.org上获取）表明，这三类中间神经元对不同的局部线索有选择性反应，以指导它们迁移到新皮质的适当区域和层。这种方法为研究天然环境中正常和异常神经元迁移开辟了一条新途径，并表明中间神经元有特定的区域和层状部署程序。
