Kozikowski Barbara A, Burt Thomas M, Tirey Debra A, Williams Lisa E, Kuzmak Barbara R, Stanton David T, Morand Kenneth L, Nelson Sandra L
Proctor & Gamble Pharmaceuticals, 8700 Mason Montgomery Road, Mason, OH 45040-8006, USA.
J Biomol Screen. 2003 Apr;8(2):205-9. doi: 10.1177/1087057103252617.
The stability of approximately 7200 compounds stored as 20-mM DMSO solutions under ambient conditions was monitored for 1 year. Compound integrity was measured by flow injection analysis using positive and negative electrospray ionization mass spectrometry. Each sample was assessed at the beginning of the study, after 12 months of storage, and at a randomized time point between the initial and final time points of the study. The relationship between length of storage and the probability of observing the compound was described by a repeated-measures logistic regression model. The probability of observing the compound was 92% after 3 months of storage at room temperature, 83% after 6 months, and 52% after 1 year in DMSO. An acceptable limit for compound loss and corresponding maximum storage time for samples in DMSO can be determined based on these results.
对约7200种以20 mM二甲基亚砜（DMSO）溶液形式在环境条件下储存的化合物的稳定性进行了为期1年的监测。使用正、负离子电喷雾电离质谱通过流动注射分析来测定化合物的完整性。在研究开始时、储存12个月后以及研究初始和最终时间点之间的一个随机时间点对每个样品进行评估。储存时间长度与观察到该化合物的概率之间的关系通过重复测量逻辑回归模型进行描述。在室温下于DMSO中储存3个月后观察到该化合物的概率为92%，6个月后为83%，1年后为52%。可根据这些结果确定化合物损失的可接受限度以及DMSO中样品相应的最长储存时间。
