Institute of Pharmaceutical Chemistry, Goethe University Frankfurt, Frankfurt, Germany.
Structural Genomics Consortium, BMLS, Goethe University Frankfurt, Frankfurt, Germany.
Methods Mol Biol. 2023;2706:59-73. doi: 10.1007/978-1-0716-3397-7_5.
The characterization of chemogenomic libraries with respect to their general effect on cellular health represents essential data for the annotation of phenotypic responses. Here, we describe a multidimensional high-content live cell assay that allows to examine cell viability in different cell lines, based on their nuclear morphology as well as modulation of small molecules of tubulin structure, mitochondrial health, and membrane integrity. The protocol monitors cells during a time course of 48 h using osteosarcoma cells, human embryonic kidney cells, and untransformed human fibroblasts as an example. The described protocol can be easily established and it can be adapted to other cell lines or other parameters important for cellular health.
关于化学基因组文库对细胞健康的普遍影响的特征描述，代表了对表型反应进行注释的重要数据。在这里，我们描述了一种多维高内涵活细胞分析，该分析基于核形态以及微管结构小分子、线粒体健康和膜完整性的调节，可用于检查不同细胞系中的细胞活力。该方案以骨肉瘤细胞、人胚肾细胞和未转化的人成纤维细胞为例，在 48 小时的时间过程中监测细胞。该描述的方案易于建立，并且可以适用于其他细胞系或对细胞健康很重要的其他参数。
